A light scatter based model relating erythrocyte vesiculation to lifetime in circulation.

Autor: Yastrebova ES; Voevodsky Institute of Chemical Kinetics and Combustion, Novosibirsk, Russian Federation., Gisich AV; Voevodsky Institute of Chemical Kinetics and Combustion, Novosibirsk, Russian Federation., Nekrasov VM; Voevodsky Institute of Chemical Kinetics and Combustion, Novosibirsk, Russian Federation., Gilev KV; Voevodsky Institute of Chemical Kinetics and Combustion, Novosibirsk, Russian Federation., Strokotov DI; Voevodsky Institute of Chemical Kinetics and Combustion, Novosibirsk, Russian Federation., Chernyshev AV; Voevodsky Institute of Chemical Kinetics and Combustion, Novosibirsk, Russian Federation., Karpenko AA; State Research Institute of Circulation Pathology, Novosibirsk, Russian Federation., Maltsev VP; Voevodsky Institute of Chemical Kinetics and Combustion, Novosibirsk, Russian Federation.; Novosibirsk State University, Novosibirsk, Russian Federation.
Jazyk: angličtina
Zdroj: Cytometry. Part A : the journal of the International Society for Analytical Cytology [Cytometry A] 2023 Sep; Vol. 103 (9), pp. 712-722. Date of Electronic Publication: 2023 May 22.
DOI: 10.1002/cyto.a.24765
Abstrakt: Methods for measuring erythrocyte age distribution are not available as a simple analytical tool. Most of them utilize the fluorescence or radioactive isotopes labeling to construct the age distribution and support physicians with aging indices of donor's erythrocytes. The age distribution of erythrocyte may be a useful snapshot of patient state over 120-days period of life. Previously, we introduced the enhanced assay of erythrocytes with measurement of 48 indices in four categories: concentration/content, morphology, aging and function (10.1002/cyto.a.24554). The aging category was formed by the indices based on the evaluation of the derived age of individual cells. The derived age does not exactly mean the real age of erythrocytes and its evaluation utilizes changes of cellular morphology during a lifespan. In this study, we are introducing the improved methodological approach that allows us to retrieve the derived age of individual erythrocytes, to construct the aging distribution, and to reform the aging category consisting of eight indices. The approach is based on the analysis of the erythrocyte vesiculation. The erythrocyte morphology is analyzed by scanning flow cytometry that measures the primary characteristics (diameter, thickness, and waist) of individual cells. The surface area (S) and sphericity index (SI) are calculated from the primary characteristics and the scattering diagram SI versus S is used in the evaluation of the derived age of each erythrocyte in a sample. We developed the algorithm to evaluate the derived age that provides eight indices in the aging category based on a model using light scatter features. The novel erythrocyte indices were measured for simulated cells and blood samples of 50 donors. We determined the first-ever reference intervals for these indices.
(© 2023 International Society for Advancement of Cytometry.)
Databáze: MEDLINE
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