Evaluation of gene expression related to immunity, apoptosis, and gut integrity that underlies Artemisia 's therapeutic effects in necrotic enteritis-challenged broilers.

Autor: El-Demerdash AS; Microbiology Department, Agriculture Research Center (ARC), Animal Health Research Institute (AHRI), Zagazig, Egypt., Mohamady SN; Clinical Pathology Department, Agriculture Research Center (ARC), Animal Health Research Institute (AHRI), Zagazig, Egypt., Megahed HM; Biochemistry Department, Agriculture Research Center (ARC), Animal Health Research Institute (AHRI), Zagazig, Egypt., Ali NM; Poultry Disease Department, Agriculture Research Center (ARC), Animal Health Research Institute (AHRI), Assuit, Egypt.
Jazyk: angličtina
Zdroj: 3 Biotech [3 Biotech] 2023 Jun; Vol. 13 (6), pp. 181. Date of Electronic Publication: 2023 May 12.
DOI: 10.1007/s13205-023-03560-9
Abstrakt: The experiment was designed to validate the effect of Artemisia annua and its novel commercial product (Navy Cox) on the control of necrotic enteritis (NE). A total of one hundred forty broiler chicks were randomly distributed into seven equal groups: G1, control negative; G2, infected with Eimeria (day 15) and C. perfringens (day 19); G3, treated with Navy Cox before challenge; G4, treated with Artemisia before challenge; G5, infected and then treated with Navy Cox; G6, infected and then treated with Artemisia; and G7, infected and treated with amoxicillin. Chicken response and immune organ indicants were recorded during the observation period (4 weeks). Whole blood and serum samples were collected for immunological evaluation, and tissue samples were collected for bacterial counts and estimation of mRNA expression of genes encoding apoptosis, tight junctions, and immunity. Chickens in the infected group revealed a significant decrease in RBCS, HB, PCV% total protein, Lysozyme, and nitric oxide activity in addition to leukocytosis, heterophilia, monocytosis, increase in cortisol, interleukins, and malondialdehyde. Treated groups displayed lower lesions, colony-forming units, and no mortality. Concurrently, a complete blood profile, antioxidants, and immune markers showed significant improvements. The mRNA expression levels of CASP, CLDN-1, OCLN, TJPI, MUC2 , and cell-mediated immune response genes ( p  < 0.0001) were significantly alleviated in the treated groups compared with the challenged counterpart. This is the first-ever report on the efficacy valuation of Navy Cox compared to standard antibiotic treatment of clostridial NE. Navy Cox proved remarkable capability to minimize C. perfringens colonization in broiler intestines, modulation of mucus production, gut health integrity, immune organs, and immune response when used as a prophylactic agent in this form or naturally as Artemisia .
Competing Interests: Conflict of interestThe authors manifested that they have no conflicts of interest.
(© King Abdulaziz City for Science and Technology 2023, Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.)
Databáze: MEDLINE
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