The Synthesis and Transfection Activity of Disulfide Polycationic Amphiphiles.

Autor: Petukhov IA; Lomonosov Institute of Fine Chemical Technologies, MIREA-Russian Technological University, 119571 Moscow, Russia., Puchkov PA; Lomonosov Institute of Fine Chemical Technologies, MIREA-Russian Technological University, 119571 Moscow, Russia., Morozova NG; Lomonosov Institute of Fine Chemical Technologies, MIREA-Russian Technological University, 119571 Moscow, Russia., Zenkova MA; Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences, 630090 Novosibirsk, Russia., Maslov MA; Lomonosov Institute of Fine Chemical Technologies, MIREA-Russian Technological University, 119571 Moscow, Russia.
Jazyk: angličtina
Zdroj: Russian journal of bioorganic chemistry [Russ J Bioorg Chem] 2023; Vol. 49 (1), pp. 41-51. Date of Electronic Publication: 2023 May 03.
DOI: 10.1134/S1068162023010235
Abstrakt: Some new polycationic amphiphiles containing a disulfide group were synthesized. Cationic liposomes formed from the compounds synthesized and a helper lipid 1,2-dioleoyl- sn -glycero-3-phosphatidylethanolamine were not toxic for HEK293 and HeLa cells and were highly effective when delivering a fluorescently labeled oligodeoxyribonucleotide. The efficacy of plasmid DNA delivery depended on the cell line and the amphiphile structure, liposomes based on tetracationic amphiphiles being the most effective transfectants. These liposomes can be used for in vitro transfection of eukaryotic cells as well as for further in vivo biological studies.
Competing Interests: The authors declare the absence of conflicts of interest.
(© Pleiades Publishing, Ltd. 2023, ISSN 1068-1620, Russian Journal of Bioorganic Chemistry, 2023, Vol. 49, No. 1, pp. 41–51. © Pleiades Publishing, Ltd., 2023.Russian Text © The Author(s), 2023, published in Bioorganicheskaya Khimiya, 2023, Vol. 49, No. 2, pp. 165–177.)
Databáze: MEDLINE
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