Clinicopathological Significance of Cancer Stem Cell Markers (OCT-3/4 and SOX-2) in Oral Submucous Fibrosis and Oral Squamous Cell Carcinoma.

Autor:	Venugopal DC; Department of Oral Medicine and Radiology, Sri Ramachandra Institute of Higher Education and Research (DU), Porur, Chennai 600116, India., Caleb CL; Department of Oral Medicine and Radiology, Sri Ramachandra Institute of Higher Education and Research (DU), Porur, Chennai 600116, India., Kirupakaran NP; Department of Oral Medicine and Radiology, Sri Ramachandra Institute of Higher Education and Research (DU), Porur, Chennai 600116, India., Shyamsundar V; Centre for Oral Cancer Prevention and Research, Sree Balaji Dental College and Hospital, Pallikaranai, Chennai 600100, India., Ravindran S; Department of Preventive Oncology (Research), Cancer Institute (WIA), Adyar, Chennai 600020, India., Yasasve M; Department of Oral Medicine and Radiology, Sri Ramachandra Institute of Higher Education and Research (DU), Porur, Chennai 600116, India., Krishnamurthy A; Department of Surgical Oncology, Cancer Institute (WIA), Adyar, Chennai 600020, India., Harikrishnan T; Department of Oral Pathology, Sri Ramachandra Institute of Higher Education and Research (DU), Porur, Chennai 600116, India., Sankarapandian S; Department of Oral Medicine and Radiology, Sri Ramachandra Institute of Higher Education and Research (DU), Porur, Chennai 600116, India., Ramshankar V; Department of Preventive Oncology (Research), Cancer Institute (WIA), Adyar, Chennai 600020, India.
Jazyk:	angličtina
Zdroj:	Biomedicines [Biomedicines] 2023 Mar 28; Vol. 11 (4). Date of Electronic Publication: 2023 Mar 28.
DOI:	10.3390/biomedicines11041040
Abstrakt:	Oral submucous fibrosis (OSMF) is highly prevalent in South East Asia with higher rates of malignant transformation in Indian subcontinent. Numerous biomarkers are now being studied to predict disease prognosis and detect malignant alterations at an early stage. Patients with clinically and biopsy-proven oral submucous fibrosis and oral squamous cell carcinoma were included in the study as the experimental group, while patients without a tobacco or betel nut habit who had their third molars surgically removed were included as the healthy control group. For the immunohistochemistry (IHC) investigation, 5-μm slices from formalin-fixed, paraffin-embedded tissue blocks (FFPE) were obtained. Fresh tissues ( n = 45) from all three groups were collected and gene expression was studied using relative quantitation-based qPCR. The protein expression of octamer-binding transcription factor 3/4 (OCT 3/4) and sex-determining region Y-box 2 (SOX 2) was evaluated in the experimental group and compared with healthy controls. The IHC results showed a significant correlation with the expression of OCT 3/4 ( p value = 0.000; χ2 = 20.244) and SOX 2 ( p value = 0.006; χ2 = 10.101) among OSCC and OSMF patients in comparison to healthy controls. Both OCT 3/4 and SOX 2 showed overexpression of four-fold and three-fold in OSMF when compared to OSCC and healthy controls, respectively. This study shows the significant importance of cancer stem cell markers OCT 3/4 and SOX 2 to assess the disease prognosis in OSMF.
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu