| Autor: |
Ríos DS; College of Science and Health Professions, Universidad Central de Bayamón, Bayamón, PR 00960, USA., Malpica-Nieves CJ; Department of Biochemistry, Universidad Central del Caribe, Bayamón, PR 00956, USA., Díaz-García A; Department of Physiology, Universidad Central del Caribe, Bayamón, PR 00956, USA., Eaton MJ; Department of Biochemistry, Universidad Central del Caribe, Bayamón, PR 00956, USA., Skatchkov SN; Department of Biochemistry, Universidad Central del Caribe, Bayamón, PR 00956, USA.; Department of Physiology, Universidad Central del Caribe, Bayamón, PR 00956, USA. |
| Abstrakt: |
Polyamines (PAs) in the nervous system has a key role in regeneration and aging. Therefore, we investigated age-related changes in the expression of PA spermidine (SPD) in the rat retina. Fluorescent immunocytochemistry was used to evaluate the accumulation of SPD in retinae from rats of postnatal days 3, 21, and 120. Glial cells were identified using glutamine synthetase (GS), whereas DAPI, a marker of cell nuclei, was used to differentiate between retinal layers. SPD localization in the retina was strikingly different between neonates and adults. In the neonatal retina (postnatal day 3-P3), SPD is strongly expressed in practically all cell types, including radial glia and neurons. SPD staining showed strong co-localization with the glial marker GS in Müller Cells (MCs) in the outer neuroblast layer. In the weaning period (postnatal day 21-P21), the SPD label was strongly expressed in all MCs, but not in neurons. In early adulthood (postnatal day 120-P120), SPD was localized in MCs only and was co-localized with the glial marker GS. A decline in the expression of PAs in neurons was observed with age while glial cells accumulated SPD after the differentiation stage (P21) and during aging in MC cellular endfoot compartments. |
