| Autor: |
Atik G; Department of Biochemistry, Faculty of Science, Ege University, Bornova, 35100 Izmir, Turkey., Kilic NM; Department of Biochemistry, Faculty of Science, Ege University, Bornova, 35100 Izmir, Turkey., Horzum N; Department of Engineering Sciences and Biocomposite Engineering Graduate Program, İzmir Katip Çelebi University, 35620 Izmir, Turkey., Odaci D; Department of Biochemistry, Faculty of Science, Ege University, Bornova, 35100 Izmir, Turkey., Timur S; Department of Biochemistry, Faculty of Science, Ege University, Bornova, 35100 Izmir, Turkey.; Central Research Test and Analysis Laboratory Application and Research Center, Ege University, Bornova, 35100 Izmir, Turkey. |
| Abstrakt: |
Multifunctional electrospun nanofibers (ENs) with improved properties have increased attention nowadays. Their insoluble forms in water with decreased hydrophobicity are desired for the immobilization of biological molecules. Also, the addition of functional groups on the backbone provides the conjugation of biomolecules onto the surface of ENs via covalent bonds to increase their stability. Here, poly(vinylidene fluoride) (PVDF) was chosen to prepare a platform, which is insoluble in water, and polyethylenimine (PEI) was used to add amine groups on the surface of ENs to bind biological molecules via covalent conjugation. So, PVDF-PEI nanofibers were prepared on a glassy carbon electrode to immobilize an antimethamphetamine antibody (Anti-METH) as a model biomolecule. The obtained PVDF-PEI/Anti-METH was used for the bioelectrochemical detection of methamphetamine (METH), a common illicit drug. Bioelectrochemical detection of METH on PVDF-PEI/Anti-METH-coated electrodes was carried out by voltammetry in the range of 2.0-50 ng/mL METH. Moreover, the effect of dansyl chloride (DNC) derivatization of METH on the sensitivity of PVDF-PEI/Anti-METH was tested. Finally, METH analysis was carried out in synthetic body fluids. The obtained results showed that PVDF-PEI ENs can be adopted as an immobilization matrix for the biorecognition elements of biobased detection systems, and the derivative of METH (METH-DNC) increased the sensitivity of PVDF-PEI/Anti-METH. |
