Tender love and disassembly: How a TLDc domain protein breaks the V-ATPase.

Autor: Wilkens S; Department of Biochemistry & Molecular Biology, SUNY Upstate Medical University, Syracuse, New York, USA., Khan MM; Department of Biochemistry & Molecular Biology, SUNY Upstate Medical University, Syracuse, New York, USA., Knight K; Department of Biochemistry & Molecular Biology, SUNY Upstate Medical University, Syracuse, New York, USA., Oot RA; Department of Biochemistry & Molecular Biology, SUNY Upstate Medical University, Syracuse, New York, USA.
Jazyk: angličtina
Zdroj: BioEssays : news and reviews in molecular, cellular and developmental biology [Bioessays] 2023 Jul; Vol. 45 (7), pp. e2200251. Date of Electronic Publication: 2023 May 15.
DOI: 10.1002/bies.202200251
Abstrakt: Vacuolar ATPases (V-ATPases, V 1 V o -ATPases) are rotary motor proton pumps that acidify intracellular compartments, and, when localized to the plasma membrane, the extracellular space. V-ATPase is regulated by a unique process referred to as reversible disassembly, wherein V 1 -ATPase disengages from V o proton channel in response to diverse environmental signals. Whereas the disassembly step of this process is ATP dependent, the (re)assembly step is not, but requires the action of a heterotrimeric chaperone referred to as the RAVE complex. Recently, an alternative pathway of holoenzyme disassembly was discovered that involves binding of Oxidation Resistance 1 (Oxr1p), a poorly characterized protein implicated in oxidative stress response. Unlike conventional reversible disassembly, which depends on enzyme activity, Oxr1p induced dissociation can occur in absence of ATP. Yeast Oxr1p belongs to the family of TLDc domain containing proteins that are conserved from yeast to mammals, and have been implicated in V-ATPase function in a variety of tissues. This brief perspective summarizes what we know about the molecular mechanisms governing both reversible (ATP dependent) and Oxr1p driven (ATP independent) V-ATPase dissociation into autoinhibited V 1 and V o subcomplexes.
(© 2023 The Authors. BioEssays published by Wiley Periodicals LLC.)
Databáze: MEDLINE
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