Copper in airborne fine particulate matter (PM 2.5 ) from urban sites causes the upregulation of pro-inflammatory cytokine IL-8 in human lung epithelial A549 cells.

Autor: Amma C; Faculty of Pharmaceutical Sciences, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University, Kakuma-Machi, Kanazawa, 920-1192, Japan., Inomata Y; Institute of Nature and Environmental Technology, Kanazawa University, Kanazawa, 920-1192, Japan., Kohno R; Faculty of Pharmaceutical Sciences, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University, Kakuma-Machi, Kanazawa, 920-1192, Japan., Satake M; Faculty of Pharmaceutical Sciences, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University, Kakuma-Machi, Kanazawa, 920-1192, Japan., Furukawa A; Faculty of Pharmaceutical Sciences, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University, Kakuma-Machi, Kanazawa, 920-1192, Japan., Nagata Y; Faculty of Pharmaceutical Sciences, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University, Kakuma-Machi, Kanazawa, 920-1192, Japan., Sugiyama H; Instrumental Analysis Division, Engineering and Technology Department, Kanazawa University, Kanazawa, 920-1192, Japan., Seto T; Faculty of Frontier Engineering, Institute of Science and Engineering, Kanazawa University, Kanazawa, 920-1192, Japan. t.seto@staff.kanazawa-u.ac.jp., Suzuki R; Faculty of Pharmaceutical Sciences, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University, Kakuma-Machi, Kanazawa, 920-1192, Japan. suzukir@p.kanazawa-u.ac.jp.
Jazyk: angličtina
Zdroj: Environmental geochemistry and health [Environ Geochem Health] 2023 Aug; Vol. 45 (8), pp. 5879-5891. Date of Electronic Publication: 2023 May 14.
DOI: 10.1007/s10653-023-01599-4
Abstrakt: Fine atmospheric particles, such as PM 2.5 , are strongly related to the onset and exacerbation of inflammatory responses leading to the development of respiratory and cardiovascular diseases. PM 2.5 is a complex mixture of tiny particles with different properties (i.e., size, morphology, and chemical components). Moreover, the mechanism by which PM 2.5 induces inflammatory responses has not been fully elucidated. Therefore, it is necessary to determine the composition of PM 2.5 to identify the main factors causing PM 2.5 -associated inflammation and diseases. In the present study, we investigated PM 2.5 from two sites (Fukue, a remote monitoring site, and Kawasaki, an urban monitoring site) with greatly different environments and PM 2.5 compositions. The results of ICP-MS and EDX-SEM indicated that PM 2.5 from Kawasaki contained more metals and significantly induced the expression of the pro-inflammatory cytokine gene IL-8 compared to the PM 2.5 from Fukue. We also verified the increased secretion of IL-8 protein from exposure to PM 2.5 from Kawasaki. We further investigated their effects on inflammatory response and cytotoxicity using metal nanoparticles (Cu, Zn, and Ni) and ions and found that the Cu nanoparticles caused a dose-dependent increase in IL-8 expression together with significant cell death. We also found that Cu nanoparticles enhanced the secretion of IL-8 protein. These results suggest that Cu in PM 2.5 is involved in lung inflammation.
(© 2023. The Author(s), under exclusive licence to Springer Nature B.V.)
Databáze: MEDLINE
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