Rapid production and free distribution of a synthetic RNA material to support SARS-CoV-2 molecular diagnostic testing.

Autor: Cleveland MH; National Institute of Standards and Technology, 100 Bureau Dr, Gaithersburg, MD, 20899, USA. Electronic address: megan.cleveland@nist.gov., Romsos EL; National Institute of Standards and Technology, 100 Bureau Dr, Gaithersburg, MD, 20899, USA., Steffen CR; National Institute of Standards and Technology, 100 Bureau Dr, Gaithersburg, MD, 20899, USA., Olson ND; National Institute of Standards and Technology, 100 Bureau Dr, Gaithersburg, MD, 20899, USA., Servetas SL; National Institute of Standards and Technology, 100 Bureau Dr, Gaithersburg, MD, 20899, USA., Valiant WG; National Institute of Standards and Technology, 100 Bureau Dr, Gaithersburg, MD, 20899, USA., Vallone PM; National Institute of Standards and Technology, 100 Bureau Dr, Gaithersburg, MD, 20899, USA.
Jazyk: angličtina
Zdroj: Biologicals : journal of the International Association of Biological Standardization [Biologicals] 2023 May; Vol. 82, pp. 101680. Date of Electronic Publication: 2023 May 03.
DOI: 10.1016/j.biologicals.2023.101680
Abstrakt: In response to the COVID-19 pandemic, the National Institute of Standards and Technology released a synthetic RNA material for SARS-CoV-2 in June 2020. The goal was to rapidly produce a material to support molecular diagnostic testing applications. This material, referred to as Research Grade Test Material 10169, was shipped free of charge to laboratories across the globe to provide a non-hazardous material for assay development and assay calibration. The material consisted of two unique regions of the SARS-CoV-2 genome approximately 4 kb nucleotides in length. The concentration of each synthetic fragment was measured using RT-dPCR methods and confirmed to be compatible with RT-qPCR methods. In this report, the preparation, stability, and limitations of this material are described.
(Published by Elsevier Ltd.)
Databáze: MEDLINE
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