| Autor: |
Vu C; Department of Biomedical Engineering, Eindhoven University of Technology, 5612 AZ Eindhoven, The Netherlands.; Institute for Complex Molecular Systems (ICMS), Eindhoven University of Technology, 5612 AZ Eindhoven, The Netherlands., Lin YT; Helia Biomonitoring, 5612 AR Eindhoven, The Netherlands., Haenen SRR; Helia Biomonitoring, 5612 AR Eindhoven, The Netherlands., Marschall J; AbSano, 5349 AB Oss, The Netherlands., Hummel A; AbSano, 5349 AB Oss, The Netherlands., Wouters SFA; AbSano, 5349 AB Oss, The Netherlands., Raats JMH; AbSano, 5349 AB Oss, The Netherlands., de Jong AM; Institute for Complex Molecular Systems (ICMS), Eindhoven University of Technology, 5612 AZ Eindhoven, The Netherlands.; Department of Applied Physics and Science Education, Eindhoven University of Technology, 5612 AZ Eindhoven, The Netherlands., Yan J; Helia Biomonitoring, 5612 AR Eindhoven, The Netherlands., Prins MWJ; Department of Biomedical Engineering, Eindhoven University of Technology, 5612 AZ Eindhoven, The Netherlands.; Institute for Complex Molecular Systems (ICMS), Eindhoven University of Technology, 5612 AZ Eindhoven, The Netherlands.; Helia Biomonitoring, 5612 AR Eindhoven, The Netherlands.; Department of Applied Physics and Science Education, Eindhoven University of Technology, 5612 AZ Eindhoven, The Netherlands. |
| Abstrakt: |
Industrial food processes are monitored to ensure that food is being produced with good quality, yield, and productivity. For developing innovative real-time monitoring and control strategies, real-time sensors are needed that can continuously report chemical and biochemical data of the manufacturing process. Here, we describe a generalizable methodology to develop affinity-based biosensors for the continuous monitoring of small molecules in industrial food processes. Phage-display antibody fragments were developed for the measurement of small molecules, as exemplified with the measurement of glycoalkaloids (GAs) in potato fruit juice. The recombinant antibodies were selected for use in a competition-based biosensor with single-molecule resolution, called biosensing by particle motion, using assay architectures with free particles as well as tethered particles. The resulting sensor measures GAs in the micromolar range, is reversible, has a measurement response time below 5 min, and enables continuous monitoring of GAs in protein-rich solutions for more than 20 h with concentration measurement errors below 15%. The demonstrated biosensor gives the perspective to enable a variety of monitoring and control strategies based on continuous measurement of small molecules in industrial food processes. |
