| Autor: |
Jažo Z; Department of Biochemistry, Faculty of Chemistry and Technology, University of Split, Ruđera Boškovića 35, 21000 Split, Croatia.; Regional Laboratory Split, Croatian Veterinary Institute, Poljička Cesta 33, 21000 Split, Croatia., Glumac M; Department of Biochemistry, Faculty of Chemistry and Technology, University of Split, Ruđera Boškovića 35, 21000 Split, Croatia.; School of Medicine, University of Split, Šoltanska 2, 21000 Split, Croatia., Paštar V; Mediterranean Institute for Life Science, Meštrovićevo Šetalište 45, 21000 Split, Croatia., Bektić S; Faculty of Sciences, University of Tuzla, Univerzitetska 4, 75000 Tuzla, Bosnia and Herzegovina., Radan M; Department of Biochemistry, Faculty of Chemistry and Technology, University of Split, Ruđera Boškovića 35, 21000 Split, Croatia., Carev I; Department of Biochemistry, Faculty of Chemistry and Technology, University of Split, Ruđera Boškovića 35, 21000 Split, Croatia.; Mediterranean Institute for Life Science, Meštrovićevo Šetalište 45, 21000 Split, Croatia.; NAOS Institute of Life Science, 355, Rue Pierre-Simon Laplace, 13290 Aix, France. |
| Abstrakt: |
In our study, we investigated the chemical composition and cytotoxic activity of essential oils isolated from Dalmatian sage ( Salvia officinalis L.) collected along the Adriatic coast of Croatia. Scanning electron microscopy (SEM) was used to examine the morphology of the stem and leaf surfaces. Essential oil excretory glands were detected on both the leaves and stem surfaces. The essential oils were isolated by hydrodistillation, and their chemical composition was determined by gas chromatography and mass spectrometry (GC-MS). Sage essential oils were mixtures of terpene compounds, among which the most common were: α - and β -thujone, camphor, and 1,8-cineol. Cytotoxic activity was tested using MTS assay on multiple cell lines: normal and immortalized fibroblasts (HF77FA and HDF-Tert), immortalized lung line (BEAS-2B), and breast adenocarcinoma (MDA-MB-231). The growth of treated cells was determined relative to control conditions without treatment. The immortalized lung line was the least resistant to the activity of the essential oils, whereas immortalized fibroblasts were the most resistant. Statistical analysis has connected the cytotoxic effect and chemical composition of the studied essential oils. To the best of our knowledge, this work is the first testing of the cytotoxic activity of S. officinalis EO's on the BEAS-2B, HF77FA, and HDF-Tert cell lines. The presented data on essential oil chemical composition and cytotoxic effect on 4 types of human cells supports pharmacotherapeutic potential this plant is known to have. |
