| Autor: |
Kopaeva MY; National Research Center 'Kurchatov Institute', 1 Akademika Kurchatova Sq., 123182 Moscow, Russia., Azieva AM; National Research Center 'Kurchatov Institute', 1 Akademika Kurchatova Sq., 123182 Moscow, Russia., Cherepov AB; National Research Center 'Kurchatov Institute', 1 Akademika Kurchatova Sq., 123182 Moscow, Russia.; Institute of General Pathology and Pathophysiology, 8 Baltiyskaya St., 125315 Moscow, Russia., Zarayskaya IY; Research Institute of Normal Physiology Named after P.K. Anokhin, 8 Baltiyskaya St., 125315 Moscow, Russia. |
| Abstrakt: |
Lactoferrin (Lf) is a multifunctional protein from the transferrin family. Of particular interest is the ability of Lf to affect a wide range of neuronal processes by modulating the expression of genes involved in long-term neuroplasticity. The expression of the immediate early gene c-fos that is rapidly activated in response to external influences, and its product, transcription factor c-Fos, is widely used as a marker of long-term neuronal plasticity. The present study aims to examine the effect of human Lf on the induction of transcription factor c-Fos in the primary mouse neuronal cultures after stimulation and to determine the cellular localization of human Lf and its colocalization with induced c-Fos protein. Primary dissociated cultures of hippocampal cells were obtained from the brains of newborn C57BL/6 mice (P0-P1). On day 7 of culturing, human Lf was added to the medium. After 24 h (day 8 in culture), c-Fos protein was induced in cells by triple application of 50 mM KCl. c-Fos content was analyzed using the immunofluorescent method 2 h after stimulation. Stimulation promoted exogenous Lf translocation into the nuclei of cultured neuronal cells, which correlated with increased induction of transcription factor c-Fos and was accompanied by nuclear colocalization of these proteins. These results attest to the potential of Lf as a modulator of neuronal processes and open up new prospects in studying the mechanisms of the regulatory effects of lactoferrin on cell function. |
