| Autor: |
Sousa LR; Experimental Pathology Laboratory, Federal University of São João del Rei (UFSJ), 400, Sebastião Gonçalves Coelho, Chanadour, Divinópolis 35501-296, MG, Brazil., Oliveira AGS; Experimental Pathology Laboratory, Federal University of São João del Rei (UFSJ), 400, Sebastião Gonçalves Coelho, Chanadour, Divinópolis 35501-296, MG, Brazil., Arantes A; Experimental Pathology Laboratory, Federal University of São João del Rei (UFSJ), 400, Sebastião Gonçalves Coelho, Chanadour, Divinópolis 35501-296, MG, Brazil., Junqueira JGM; Institute of Chemistry, Federal University of Goiás (UFG), University Campus, Goiânia 74968-755, GO, Brazil., Alexandre GP; Institute of Chemistry, Federal University of Goiás (UFG), University Campus, Goiânia 74968-755, GO, Brazil., Severino VGP; Institute of Chemistry, Federal University of Goiás (UFG), University Campus, Goiânia 74968-755, GO, Brazil., Reis RM; Molecular Oncology Research Center, Barretos Cancer Hospital, Barretos 14784-400, SP, Brazil.; Life and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, 4710-057 Braga, Portugal.; ICVS/3B's-PT Government Associate Laboratory, 4710-057 Braga, Portugal.; ICVS/3B's-PT Government Associate Laboratory, 4805-017 Guimarães, Portugal., Kim B; College of Medicine, Kyung Hee University, Seoul 02453, Republic of Korea., Ribeiro RIMA; Experimental Pathology Laboratory, Federal University of São João del Rei (UFSJ), 400, Sebastião Gonçalves Coelho, Chanadour, Divinópolis 35501-296, MG, Brazil. |
| Abstrakt: |
Glioblastoma (GBM) is an incurable primary brain tumor with a poor prognosis. Resection, radiation therapy, and temozolomide (TMZ) are insufficient to increase survival, making the treatment limited. Thus, the search for more effective and specific treatments is essential, making plants a promising source for elucidating new anti-glioblastoma compounds. Accordingly, this study investigated the effects of four fractions of hexane and ethyl acetate extract of Annona coriacea Mart., enriched with acetogenins, against GBM cell lines. All four fractions were selectively cytotoxic to GBM cells when compared to TMZ. Moreover, A. coriacea fractions delayed cell migration; reduced cytoplasmic projections, the metalloproteinase 2 (MMP-2) activity; and induced morphological changes characteristic of necroptosis, possibly correlated with the increase in receptor-interacting protein kinase 1 and 3 (RIP-1 and RIP-3), apoptosis-inducing factor (AIF), and the non-activation of cleaved caspase 8. The present findings reinforce that fractions of A. coriacea Mart. should be considered for more studies focusing treatment of GBM. |
