Redox perturbations in yeast cells lacking glutathione reductase.

Autor: Janeczko A; Department of Biology, Institute of Biology and Biotechnology, College of Natural Sciences, University of Rzeszow, Rzeszow, Poland., Przywara M; Department of Biology, Institute of Biology and Biotechnology, College of Natural Sciences, University of Rzeszow, Rzeszow, Poland., Maslanka R; Department of Biology, Institute of Biology and Biotechnology, College of Natural Sciences, University of Rzeszow, Rzeszow, Poland., Raś B; Department of Biology, Institute of Biology and Biotechnology, College of Natural Sciences, University of Rzeszow, Rzeszow, Poland., Ziaja K; Department of Biology, Institute of Biology and Biotechnology, College of Natural Sciences, University of Rzeszow, Rzeszow, Poland., Kwolek-Mirek M; Department of Biology, Institute of Biology and Biotechnology, College of Natural Sciences, University of Rzeszow, Rzeszow, Poland., Zadrag-Tecza R; Department of Biology, Institute of Biology and Biotechnology, College of Natural Sciences, University of Rzeszow, Rzeszow, Poland. Electronic address: retecza@ur.edu.pl., Bednarska S; Department of Biology, Institute of Biology and Biotechnology, College of Natural Sciences, University of Rzeszow, Rzeszow, Poland.
Jazyk: angličtina
Zdroj: Fungal genetics and biology : FG & B [Fungal Genet Biol] 2023 Jun; Vol. 167, pp. 103810. Date of Electronic Publication: 2023 May 10.
DOI: 10.1016/j.fgb.2023.103810
Abstrakt: Cellular redox homeostasis has a major effect on cell functions and its maintenance is supported by glutathione and protein thiols which serve as redox buffers in cells. The regulation of the glutathione biosynthetic pathway is a focus of a lot of scientific research. However, still little is known about how complex cellular networks influence glutathione homeostasis. In this work was used an experimental system based on an S. cerevisiae yeast mutant with a lack of the glutathione reductase enzyme and allyl alcohol as a precursor of acrolein inside the cell to determine the cellular processes influencing glutathione homeostasis. The absence of Glr1p slows down the growth rate of the cell population, especially in the presence of allyl alcohol, but does not lead to complete inhibition of the cell's reproductive capacity. It also amends the GSH/GSSG ratio and the share of NADPH and NADP + in the total NADP(H) pool. The obtained results show that potential pathways involved in the maintenance of redox homeostasis are based from one side on de novo synthesis of GSH as indicated by increased activity of γ-GCS and increased expression of GSH1 gene in the Δglr1 mutant, from the other hand, on increased the level of NADPH. This is because the lower ratio of GSH/GSSG can be counterbalanced with the NADPH/NADP + alternative system. The higher level of NADPH can be used by the thioredoxin system and other enzymes requiring NADPH to reduce cytosolic GSSG and maintain glutathione redox potential.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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