Chemical Composition, Anthelmintic Activity, and Mechanism of Action of Lippia dominguensis Mold. Essential Oil on Haemonchus contortus.

Autor: Espino Ureña MJ; Department of Pathology and Parasitology, Institute of Biomedical Sciences, Federal University of Alfenas, Gabriel Monteiro da Silva St., 700, 37130-001, Alfenas, Minas Gerais, Brazil.; Animal Production Center, Dominican Institute of Agricultural and Forestry Research, Duarte Av. Km 24, Pedro Brand, 340 parcel, Santo Domingo., Dominican Republic.; Faculty of Agronomic and Veterinary Sciences, Autonomous University of Santo Domingo, Camino de Engombe St., 10904, Santo Domingo, Dominican Republic., Katchborian-Neto A; Institute of Chemistry, Federal University of Alfenas, Gabriel Monteiro da Silva St., 700, 37130-001, Alfenas, Minas Gerais, Brazil., Trinidad AB; Animal Production Center, Dominican Institute of Agricultural and Forestry Research, Duarte Av. Km 24, Pedro Brand, 340 parcel, Santo Domingo., Dominican Republic., Ramírez Ramírez M; Institute of Chemistry, Science Faculty, Autonomous University of Santo Domingo, Alma Mater Av. Zona Universitaria, Santo Domingo, Dominican Republic., Vásquez Tineo M; Institute of Chemistry, Science Faculty, Autonomous University of Santo Domingo, Alma Mater Av. Zona Universitaria, Santo Domingo, Dominican Republic., Vilemar de Araújo-Filho J; Laboratory of Parasitic Diseases, Faculty of Veterinary, State University of Ceará, Dr. Silas Munguba Av., 1700, 60714-903, Fortaleza, Ceará, Brazil., Ribeiro WLC; Department of Physiology and Pharmacology, Federal University of Ceará, Coronel Nunes deMelo St., 1127, 60430-275, Rodolfo Teófilo, Fortaleza, CE, Brazil., de Souza Collares Maia Castelo-Branco D; Department of Pathology and Legal Medicine, School of Medicine, Federal University of Ceará, Alexandre Baraúna St., 949, 60430-160, Rodolfo Teófilo, Fortaleza, CE, Brazil., de Oliveira LMB; Laboratory of Parasitic Diseases, Faculty of Veterinary, State University of Ceará, Dr. Silas Munguba Av., 1700, 60714-903, Fortaleza, Ceará, Brazil., Bevilaqua CML; Laboratory of Parasitic Diseases, Faculty of Veterinary, State University of Ceará, Dr. Silas Munguba Av., 1700, 60714-903, Fortaleza, Ceará, Brazil., Chagas-Paula DA; Institute of Chemistry, Federal University of Alfenas, Gabriel Monteiro da Silva St., 700, 37130-001, Alfenas, Minas Gerais, Brazil., Viegas C Jr; Laboratory of Research in Medicinal Chemistry - PeQuiM, Institute of Chemistry, Federal University of Alfenas, Jovino Fernandes Sales Ave., 2600, 37133-840, Alfenas, Minas Gerais, Brazil., Marques MJ; Department of Pathology and Parasitology, Institute of Biomedical Sciences, Federal University of Alfenas, Gabriel Monteiro da Silva St., 700, 37130-001, Alfenas, Minas Gerais, Brazil.
Jazyk: angličtina
Zdroj: Chemistry & biodiversity [Chem Biodivers] 2023 Jul; Vol. 20 (7), pp. e202300135. Date of Electronic Publication: 2023 Jun 16.
DOI: 10.1002/cbdv.202300135
Abstrakt: Gastrointestinal nematode parasitism is a major burden to small ruminant production globally, compounded by increasing anthelmintic resistance. Previous studies have identified essential oils (EOs) from the Lippia genus with antiprotozoal and anthelmintic effects. Lippia dominguensis Moldenke (Ld), an endemic specie from the Dominican Republic, has similar popular uses, however, is chemically and pharmacologically yet uncharacterized. Here, we investigated the in vitro anthelmintic activity of LdEO and its ultrastructural effects on eggs and adult nematodes of Haemonchus contortus multidrug-resistant isolated. The GC/MS analysis showed linalool (33.85 %), 1,8-cineole (30.88 %), and δ-terpineol (10.61 %) as the main EO constituents. The LdEO showed an IC 50 =0.523 mg/mL in the egg hatch test, and the motility in the adult worm motility test was 95.8 % at 1 mg/mL. The confocal scanning laser microscopy of eggs indicated permeabilization or disruption of egg cell membranes as the possible mechanism of action of LdEO. The scanning electron microscopy of adult worms showed wrinkling, undulations, and cuticular disruptions. The LdEO displayed significant in vitro anthelmintic activity on eggs and adult worms of H. contortus. Additionally, the LdEO showed low oral toxicity in mice at 2,000 mg/kg. Thus, additional in vivo studies are justified to determine its anthelmintic efficacy in small ruminants.
(© 2023 Wiley-VHCA AG, Zurich, Switzerland.)
Databáze: MEDLINE
Externí odkaz: