Determination of 24,25-dihydroxyvitamin D 3 in Vitamin D External Quality Assessment Scheme samples using a reference measurement procedure.

Autor: Wise SA; Office of Dietary Supplements, National Institutes of Health, Bethesda, MD 20892, USA. Electronic address: stephen.wise@nih.gov., Hahm G; National Institute of Standards and Technology (NIST), Gaithersburg, MD 20899, USA., Burdette CQ; National Institute of Standards and Technology (NIST), Gaithersburg, MD 20899, USA., Tai SS; National Institute of Standards and Technology (NIST), Gaithersburg, MD 20899, USA., Camara JE; National Institute of Standards and Technology (NIST), Gaithersburg, MD 20899, USA., Sempos CT; Vitamin D Standardization Program LLC, Havre de Grace, MD 21078, USA., Williams EL; Imperial Healthcare NHS Trust, London W6 8RF, UK.
Jazyk: angličtina
Zdroj: The Journal of steroid biochemistry and molecular biology [J Steroid Biochem Mol Biol] 2023 Jul; Vol. 231, pp. 106318. Date of Electronic Publication: 2023 May 09.
DOI: 10.1016/j.jsbmb.2023.106318
Abstrakt: Ninety archived human serum samples from the Vitamin D External Quality Assessment Scheme (DEQAS) were analyzed using a reference measurement procedure (RMP) based on isotope dilution liquid chromatography - tandem mass spectrometry (ID LC-MS/MS) for the determination of 24,25-dihydroxyvitamin D 3 [24,25(OH) 2 D 3 ]. These 24,25(OH) 2 D 3 results, in conjunction with concentration values assigned using RMPs for 25-hydroxyvitamin D 2 [25(OH)D 2 ] and 25-hydroxyvitamin D 3 [25(OH)D 3 ], provide a valuable resource for assessing the accuracy of measurements for 24,25(OH) 2 D 3 and for investigating the relationship between 24,25(OH) 2 D 3 and 25(OH)D 3 . Results for 24,25(OH) 2 D 3 using the RMP were compared to DEQAS consensus values demonstrating that the consensus values were not sufficient to assess the accuracy of measurements among different laboratories and methods. A multivariable regression analysis approach using historical DEQAS consensus values for various total 25(OH)D assays was used to assess the contribution of 24,25(OH) 2 D 3 concentration on the assay response. The response of several ligand binding assays for total 25(OH)D was shown to be impacted by the presence of 24,25(OH) 2 D 3 .
Competing Interests: Declarations of Competing Interest The authors declare that they have no competing financial interests.
(Published by Elsevier Ltd.)
Databáze: MEDLINE
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