Mechanisms of skin vascular maturation and maintenance captured by longitudinal imaging of live mice.

Autor:	Kam CY; Department of Genetics, Yale School of Medicine, New Haven, CT 06510, USA., Singh ID; Department of Genetics, Yale School of Medicine, New Haven, CT 06510, USA., Gonzalez DG; Department of Genetics, Yale School of Medicine, New Haven, CT 06510, USA., Matte-Martone C; Department of Genetics, Yale School of Medicine, New Haven, CT 06510, USA., Solá P; Institute for Research in Biomedicine (IRB Barcelona), The Barcelona Institute of Science and Technology, 08028 Barcelona, Spain., Solanas G; Institute for Research in Biomedicine (IRB Barcelona), The Barcelona Institute of Science and Technology, 08028 Barcelona, Spain., Bonjoch J; Institute for Research in Biomedicine (IRB Barcelona), The Barcelona Institute of Science and Technology, 08028 Barcelona, Spain., Marsh E; Department of Genetics, Yale School of Medicine, New Haven, CT 06510, USA., Hirschi KK; Department of Cell Biology, University of Virginia School of Medicine, Charlottesville, VA 22908, USA. Electronic address: kkh4yy@virginia.edu., Greco V; Department of Genetics, Yale School of Medicine, New Haven, CT 06510, USA; Departments of Cell Biology and Dermatology, Yale Stem Cell Center, Yale Cancer Center, Yale School of Medicine, New Haven, CT 06510, USA. Electronic address: valentina.greco@yale.edu.
Jazyk:	angličtina
Zdroj:	Cell [Cell] 2023 May 25; Vol. 186 (11), pp. 2345-2360.e16. Date of Electronic Publication: 2023 May 10.
DOI:	10.1016/j.cell.2023.04.017
Abstrakt:	A functional network of blood vessels is essential for organ growth and homeostasis, yet how the vasculature matures and maintains homeostasis remains elusive in live mice. By longitudinally tracking the same neonatal endothelial cells (ECs) over days to weeks, we found that capillary plexus expansion is driven by vessel regression to optimize network perfusion. Neonatal ECs rearrange positions to evenly distribute throughout the developing plexus and become positionally stable in adulthood. Upon local ablation, adult ECs survive through a plasmalemmal self-repair response, while neonatal ECs are predisposed to die. Furthermore, adult ECs reactivate migration to assist vessel repair. Global ablation reveals coordinated maintenance of the adult vascular architecture that allows for eventual network recovery. Lastly, neonatal remodeling and adult maintenance of the skin vascular plexus are orchestrated by temporally restricted, neonatal VEGFR2 signaling. Our work sheds light on fundamental mechanisms that underlie both vascular maturation and adult homeostasis in vivo. Competing Interests: Declaration of interests The authors declare no competing financial interests. (Copyright © 2023 Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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