Orientation of capture antibodies on gold nanoparticles to improve the sensitivity of ELISA-based medical devices.

Autor: Susini V; Department of Translational Research and of New Surgical and Medical Technologies, University of Pisa, Via Savi 10, Pisa, 56126, Italy. Electronic address: vanessa.susini@med.unipi.it., Ferraro G; Department of Chemistry 'Ugo Schiff' & Center for Colloid and Surface Science (CSGI), University of Florence, Via Della Lastruccia 3, Sesto Fiorentino, 50019, Florence, Italy., Fierabracci V; Department of Translational Research and of New Surgical and Medical Technologies, University of Pisa, Via Savi 10, Pisa, 56126, Italy., Ursino S; Department of Translational Research and of New Surgical and Medical Technologies, University of Pisa, Via Savi 10, Pisa, 56126, Italy., Sanguinetti C; Department of Translational Research and of New Surgical and Medical Technologies, University of Pisa, Via Savi 10, Pisa, 56126, Italy., Caponi L; Department of Translational Research and of New Surgical and Medical Technologies, University of Pisa, Via Savi 10, Pisa, 56126, Italy., Romiti N; Department of Translational Research and of New Surgical and Medical Technologies, University of Pisa, Via Savi 10, Pisa, 56126, Italy., Rossi VL; BioMérieux Italia S.p.a., Via di Campigliano 58, Bagno a Ripoli, 50012, Florence, Italy., Sanesi A; BioMérieux Italia S.p.a., Via di Campigliano 58, Bagno a Ripoli, 50012, Florence, Italy., Paolicchi A; Department of Translational Research and of New Surgical and Medical Technologies, University of Pisa, Via Savi 10, Pisa, 56126, Italy., Franzini M; Department of Translational Research and of New Surgical and Medical Technologies, University of Pisa, Via Savi 10, Pisa, 56126, Italy., Fratini E; Department of Chemistry 'Ugo Schiff' & Center for Colloid and Surface Science (CSGI), University of Florence, Via Della Lastruccia 3, Sesto Fiorentino, 50019, Florence, Italy.
Jazyk: angličtina
Zdroj: Talanta [Talanta] 2023 Aug 01; Vol. 260, pp. 124650. Date of Electronic Publication: 2023 May 05.
DOI: 10.1016/j.talanta.2023.124650
Abstrakt: The sensitivity of ELISA-based devices strongly depends on the right orientation of antibodies on the sensor surface. The aim of this work was to increase the analytical performance of a commercial ELISA-based medical device (VIDAS®), thanks to the specific orientation of antibodies on gold nanostructured disposables. For this purpose, fPSA VIDAS® assay was used as model and the disposable providing the antigen binding surface (SPR®) was functionalized with gold nanostructures coated with monovalent half-fragment antibodies (reduced IgG, rIgG). The functionalization of polystyrene SPRs® with gold nanostructures was achieved through a one-step incubation of gold dispersions in a mixture of non-toxic solvents. Five different concentrations of gold nanoparticles (NPs) were tested with a maximum fluorescence enhancement for NPs density around 3-8 *10 3 NPs/μm 2 (752 ± 11 RFV vs 316 ± 5 RFV of bare SPRs®). The comparison of the dose-response curve obtained with commercial and gold coated-SPRs® revealed a significant improvement (p < 0.0001) of the analytical sensitivity of the VIDAS® system using nanostructured disposables. This improved version of SPRs® allows to distinguish small variations of fPSA concentrations opening the way to the application of this biomarker to other kinds of cancer as recently described in the literature.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023. Published by Elsevier B.V.)
Databáze: MEDLINE
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