Replisome-cohesin interactions provided by the Tof1-Csm3 and Mrc1 cohesion establishment factors.

Autor: Shrestha S; Chromosome Segregation Laboratory, The Francis Crick Institute, London, NW1 1AT, UK., Minamino M; Chromosome Segregation Laboratory, The Francis Crick Institute, London, NW1 1AT, UK., Chen ZA; Bioanalytics Unit, Institute of Biotechnology, Technische Universität Berlin, 13355, Berlin, Germany., Bouchoux C; Chromosome Segregation Laboratory, The Francis Crick Institute, London, NW1 1AT, UK., Rappsilber J; Bioanalytics Unit, Institute of Biotechnology, Technische Universität Berlin, 13355, Berlin, Germany.; Wellcome Centre for Cell Biology, University of Edinburgh, Edinburgh, EH9 3BF, UK., Uhlmann F; Chromosome Segregation Laboratory, The Francis Crick Institute, London, NW1 1AT, UK. frank.uhlmann@crick.ac.uk.
Jazyk: angličtina
Zdroj: Chromosoma [Chromosoma] 2023 Jun; Vol. 132 (2), pp. 117-135. Date of Electronic Publication: 2023 May 11.
DOI: 10.1007/s00412-023-00797-4
Abstrakt: The chromosomal cohesin complex establishes sister chromatid cohesion during S phase, which forms the basis for faithful segregation of DNA replication products during cell divisions. Cohesion establishment is defective in the absence of either of three non-essential Saccharomyces cerevisiae replication fork components Tof1-Csm3 and Mrc1. Here, we investigate how these conserved factors contribute to cohesion establishment. Tof1-Csm3 and Mrc1 serve known roles during DNA replication, including replication checkpoint signaling, securing replication fork speed, as well as recruiting topoisomerase I and the histone chaperone FACT. By modulating each of these functions independently, we rule out that one of these known replication roles explains the contribution of Tof1-Csm3 and Mrc1 to cohesion establishment. Instead, using purified components, we reveal direct and multipronged protein interactions of Tof1-Csm3 and Mrc1 with the cohesin complex. Our findings open the possibility that a series of physical interactions between replication fork components and cohesin facilitate successful establishment of sister chromatid cohesion during DNA replication.
(© 2023. The Author(s).)
Databáze: MEDLINE
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