Fine resolution analysis of bacterial communities associated with Neochloris oleoabundans culture and insights into terpenes as contamination control agents.

Autor: Molina-Aulestia DT; Bioprocess Engineering and Biotechnology Department, Federal University of Paraná Curitiba, Curitiba, 81531-980, PR, Brazil., de Carvalho JC; Bioprocess Engineering and Biotechnology Department, Federal University of Paraná Curitiba, Curitiba, 81531-980, PR, Brazil. jccarvalho@ufpr.br., de Melo Pereira GV; Bioprocess Engineering and Biotechnology Department, Federal University of Paraná Curitiba, Curitiba, 81531-980, PR, Brazil., da Silva Vale A; Bioprocess Engineering and Biotechnology Department, Federal University of Paraná Curitiba, Curitiba, 81531-980, PR, Brazil., de Carvalho DP; Federal Institute of Education, Science and Technology of Paraná (IFPR), Londrina, PR, Brazil., Soccol VT; Bioprocess Engineering and Biotechnology Department, Federal University of Paraná Curitiba, Curitiba, 81531-980, PR, Brazil., Soccol CR; Bioprocess Engineering and Biotechnology Department, Federal University of Paraná Curitiba, Curitiba, 81531-980, PR, Brazil.
Jazyk: angličtina
Zdroj: World journal of microbiology & biotechnology [World J Microbiol Biotechnol] 2023 May 11; Vol. 39 (7), pp. 192. Date of Electronic Publication: 2023 May 11.
DOI: 10.1007/s11274-023-03641-0
Abstrakt: Biological contamination is one of the main bottlenecks in microalgae production, reducing quality and productivity and sometimes leading to the complete loss of the cultures. Selecting terpenes can be a pathway toward eco-friendly contamination control in microalgae cultures. This work evaluated the presence of bacterial contaminants in N. oleoabundans cultures through HTS and 16 S analysis and their susceptibility to six natural terpenes (α-pinene, β-pinene, limonene, trans-cinnamaldehyde, linalool, and eugenol). The principal phyla identified were Proteobacteria, Bacteroidetes, and Actinobacteria, and based on these data, 89 bacterial isolates of seven genera were obtained (36 Aureimonas sp., 27 Microbacterium sp., 5 Pseudomonas sp., 9 Bacillus sp., 14 Shinella sp., 1 Brevundimonas sp., and 1 Exiguobacterium sp.) at 25ºC in the presence of light. It was possible to observe that Beta-pinene 50 mg L - 1 only inhibited Bacillus sp. In contrast, Alpha-pinene, Linalool, and Trans-cinnamaldehyde, at a concentration of 6.25 mg L - 1 efficiently inhibited most isolates. The inhibition percentages found were 79-99%.
(© 2023. The Author(s), under exclusive licence to Springer Nature B.V.)
Databáze: MEDLINE
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