Optimizing Fe Nutrition for Algal Growth.

Autor:	Glaesener AG; California Institute for Quantitative Biosciences (QB3), University of California, Berkeley, CA, USA., Merchant SS; California Institute for Quantitative Biosciences (QB3), University of California, Berkeley, CA, USA. sabeeha@berkeley.edu.; Department of Molecular and Cell Biology, University of California, Berkeley, CA, USA. sabeeha@berkeley.edu.; Department of Plant and Microbial Biology, University of California, Berkeley, CA, USA. sabeeha@berkeley.edu.; Lawrence Livermore National Laboratory, Physical and Life Science Directorate, Livermore, CA, USA. sabeeha@berkeley.edu.
Jazyk:	angličtina
Zdroj:	Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2023; Vol. 2665, pp. 203-215.
DOI:	10.1007/978-1-0716-3183-6_16
Abstrakt:	Chlamydomonas is an excellent reference system for dissecting the impact of iron (Fe) nutrition on photosynthetic and other metabolisms. The operational definition of four stages of Fe nutrition is described and a guide to the practical use of these stages is offered, specifically the preparation of media and growth of mixotrophic cultures. A key consideration is the impact of carbon metabolism on the expression of Fe-containing enzymes and hence the Fe quota. The absolute concentration of Fe in the medium is less determinative of gene expression than the Fe available on a per-cell basis. In nature, algal cells may transition from Fe-replete to -deficient to -limited during a bloom. (© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze:	MEDLINE
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