Towards epigenetic regulation of triple-negative breast cancer via ligand-mediated nanoparticles.

Autor:	Picheth GF; Department of Basic Pathology, Federal University of Paraná, Curitiba, Paraná, Brazil.; Department of Biochemistry, Federal University of Paraná, Curitiba, Paraná, 8150-000, Brazil., Cardoso GC; Department of Basic Pathology, Federal University of Paraná, Curitiba, Paraná, Brazil., Collini MB; Department of Basic Pathology, Federal University of Paraná, Curitiba, Paraná, Brazil., Filizzola JO; Department of Basic Pathology, Federal University of Paraná, Curitiba, Paraná, Brazil., Colauto LB; Department of Basic Pathology, Federal University of Paraná, Curitiba, Paraná, Brazil., Nunes GG; Department of Chemistry, Federal University of Paraná, Curitiba, Paraná, 8150-000, Brazil., Pirich CL; Department of Bioprocess Engineering & Biotechnology, Federal University of Paraná, Curitiba, Paraná, 8150-000, Brazil., da Silva TA; Department of Bioprocess Engineering & Biotechnology, Federal University of Paraná, Curitiba, Paraná, 8150-000, Brazil., Pontes FL; Department of Biochemistry, Federal University of Paraná, Curitiba, Paraná, 8150-000, Brazil., Zattoni IF; School of Medicine, Pontifical Catholic University of Paraná. Curitiba, Paraná, 80215-901, Brazil., Cavalieri EA; Department of Basic Pathology, Federal University of Paraná, Curitiba, Paraná, Brazil., Klassen G; Department of Basic Pathology, Federal University of Paraná, Curitiba, Paraná, Brazil.
Jazyk:	angličtina
Zdroj:	Nanomedicine (London, England) [Nanomedicine (Lond)] 2023 Mar; Vol. 18 (6), pp. 541-554. Date of Electronic Publication: 2023 May 11.
DOI:	10.2217/nnm-2023-0006
Abstrakt:	Aims: Develop and analyze triple-negative breast cancer targeted nanoparticles loaded with the demethylating agent decitabine. Materials & methods: The polymers were synthesized by ring-opening polymerization of D,L-lactide and formulated into nanoparticles via emulsion-evaporation method. The nanoparticles were characterized by physicochemical analysis as well as in vitro using breast cancer cell lineages. Results & conclusion: The targeted nanoparticles exhibited a hydrodynamic diameter of 75 ± 12 nm, zeta potential -6.3 ± 0.2 mV and spherical morphology, and displayed greater in vitro accumulation into MDA-MB-231 (triple-negative breast cancer cell-line) compared with MCF7 and HB4A cell lineages as verified by fluorescence confocal microscopy and significant demethylating effects via ADAM33 screening by PCR.
Databáze:	MEDLINE
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