Label-free intraoperative nerve detection and visualization using ratiometric diffuse reflectance spectroscopy.

Autor: Throckmorton GA; Department of Biomedical Engineering, Vanderbilt University, Nashville, TN, 37235, USA.; Vanderbilt Biophotonics Center, Vanderbilt University, Nashville, TN, 37235, USA., Haugen E; Department of Biomedical Engineering, Vanderbilt University, Nashville, TN, 37235, USA.; Vanderbilt Biophotonics Center, Vanderbilt University, Nashville, TN, 37235, USA., Thomas G; Department of Biomedical Engineering, Vanderbilt University, Nashville, TN, 37235, USA.; Vanderbilt Biophotonics Center, Vanderbilt University, Nashville, TN, 37235, USA., Willmon P; Department of Biomedical Engineering, Vanderbilt University, Nashville, TN, 37235, USA.; Vanderbilt Biophotonics Center, Vanderbilt University, Nashville, TN, 37235, USA., Baba JS; Yaya Scientific LLC, Nashville, TN, 37203, USA., Solórzano CC; Department of Surgery, Vanderbilt University Medical Center, Nashville, TN, 37232, USA., Mahadevan-Jansen A; Department of Biomedical Engineering, Vanderbilt University, Nashville, TN, 37235, USA. anita.mahadevan-jansen@vanderbilt.edu.; Vanderbilt Biophotonics Center, Vanderbilt University, Nashville, TN, 37235, USA. anita.mahadevan-jansen@vanderbilt.edu.; Department of Surgery, Vanderbilt University Medical Center, Nashville, TN, 37232, USA. anita.mahadevan-jansen@vanderbilt.edu.; Department of Otolaryngology, Vanderbilt University Medical Center, Nashville, TN, 37232, USA. anita.mahadevan-jansen@vanderbilt.edu.; Department of Neurological Surgery, Vanderbilt University Medical Center, Nashville, TN, 37232, USA. anita.mahadevan-jansen@vanderbilt.edu.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2023 May 10; Vol. 13 (1), pp. 7599. Date of Electronic Publication: 2023 May 10.
DOI: 10.1038/s41598-023-34054-6
Abstrakt: Iatrogenic nerve injuries contribute significantly to postoperative morbidity across various surgical disciplines and occur in approximately 500,000 cases annually in the US alone. Currently, there are no clinically adopted means to intraoperatively visualize nerves beyond the surgeon's visual assessment. Here, we report a label-free method for nerve detection using diffuse reflectance spectroscopy (DRS). Starting with an in vivo rat model, fiber- and imaging-based DRS independently identified similar wavelengths that provided optimal contrast for nerve identification with an accuracy of 92%. Optical property measurements of rat and human cadaver tissues verify that the source of contrast between nerve and surrounding tissues is largely due to higher scattering in nerve and differences in oxygenated hemoglobin content. Clinical feasibility was demonstrated in patients undergoing thyroidectomies using both probe-based and imaging-based approaches where the nerve were identified with 91% accuracy. Based on our preliminary results, DRS has the potential to both provide surgeons with a label-free, intraoperative means of nerve visualization and reduce the incidence of iatrogenic nerve injuries along with its detrimental complications.
(© 2023. The Author(s).)
Databáze: MEDLINE
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