Characterization of three cholinergic inputs to the cochlear nucleus.

Autor: Beebe NL; Hearing Research Group, Department of Anatomy and Neurobiology, Northeast Ohio Medical University, Rootstown, OH, USA., Herrera YN; Kresge Hearing Research Institute, Department of Otolaryngology-Head and Neck Surgery, University of Michigan, Ann Arbor, MI, USA., Noftz WA; Hearing Research Group, Department of Anatomy and Neurobiology, Northeast Ohio Medical University, Rootstown, OH, USA., Roberts MT; Kresge Hearing Research Institute, Department of Otolaryngology-Head and Neck Surgery, University of Michigan, Ann Arbor, MI, USA; Department of Molecular and Integrative Physiology, University of Michigan, Ann Arbor, MI, USA., Schofield BR; Hearing Research Group, Department of Anatomy and Neurobiology, Northeast Ohio Medical University, Rootstown, OH, USA. Electronic address: bschofie@neomed.edu.
Jazyk: angličtina
Zdroj: Journal of chemical neuroanatomy [J Chem Neuroanat] 2023 Sep; Vol. 131, pp. 102284. Date of Electronic Publication: 2023 May 08.
DOI: 10.1016/j.jchemneu.2023.102284
Abstrakt: Acetylcholine modulates responses throughout the auditory system, including at the earliest brain level, the cochlear nucleus (CN). Previous studies have shown multiple sources of cholinergic input to the CN but information about their relative contributions and the distribution of inputs from each source is lacking. Here, we used staining for cholinergic axons and boutons, retrograde tract tracing, and acetylcholine-selective anterograde tracing to characterize three sources of acetylcholine input to the CN in mice. Staining for cholinergic axons showed heavy cholinergic inputs to granule cell areas and the dorsal CN with lighter input to the ventral CN. Retrograde tract tracing revealed that cholinergic cells from the superior olivary complex, pontomesencephalic tegmentum, and lateral paragigantocellular nucleus send projections to the CN. When we selectively labeled cholinergic axons from each source to the CN, we found surprising similarities in their terminal distributions, with patterns that were overlapping rather than complementary. Each source heavily targeted granule cell areas and the dorsal CN (especially the deep dorsal CN) and sent light input into the ventral CN. Our results demonstrate convergence of cholinergic inputs from multiple sources in most regions of the CN and raise the possibility of convergence onto single CN cells. Linking sources of acetylcholine and their patterns of activity to modulation of specific cell types in the CN will be an important next step in understanding cholinergic modulation of early auditory processing.
Competing Interests: Declaration of Competing Interest none
(Copyright © 2023 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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