Integration of CuS/ZnIn 2 S 4 flower-like heterojunctions and (MnCo)Fe 2 O 4 nanozyme for signal amplification and their application to ultrasensitive PEC aptasensing of cancer biomarker.

Autor: Yang HY; Key Laboratory of the Ministry of Education for Advanced Catalysis Materials, College of Chemistry and Materials Science, College of Geography and Environmental Sciences, Zhejiang Normal University, Jinhua, 321004, China., Wei JJ; Key Laboratory of the Ministry of Education for Advanced Catalysis Materials, College of Chemistry and Materials Science, College of Geography and Environmental Sciences, Zhejiang Normal University, Jinhua, 321004, China., Zheng JY; Key Laboratory of the Ministry of Education for Advanced Catalysis Materials, College of Chemistry and Materials Science, College of Geography and Environmental Sciences, Zhejiang Normal University, Jinhua, 321004, China., Ai QY; Key Laboratory of the Ministry of Education for Advanced Catalysis Materials, College of Chemistry and Materials Science, College of Geography and Environmental Sciences, Zhejiang Normal University, Jinhua, 321004, China., Wang AJ; Key Laboratory of the Ministry of Education for Advanced Catalysis Materials, College of Chemistry and Materials Science, College of Geography and Environmental Sciences, Zhejiang Normal University, Jinhua, 321004, China. Electronic address: ajwang@zjnu.cn., Feng JJ; Key Laboratory of the Ministry of Education for Advanced Catalysis Materials, College of Chemistry and Materials Science, College of Geography and Environmental Sciences, Zhejiang Normal University, Jinhua, 321004, China. Electronic address: jjfeng@zjnu.cn.
Jazyk: angličtina
Zdroj: Talanta [Talanta] 2023 Aug 01; Vol. 260, pp. 124631. Date of Electronic Publication: 2023 May 03.
DOI: 10.1016/j.talanta.2023.124631
Abstrakt: Vascular endothelial growth factor 165 (VEGF 165 ) is a crucial regulator of angiogenesis and works as a major protein biomarker of cancer metastasis. Therefore, its quantitative detection is pivotal in clinic. In this work, CuS/ZnIn 2 S 4 flower-like heterojunctions had strong and stable photocurrents, which behaved as photoactive material to construct a photoelectrochemical (PEC) aptasensor for detecting VEGF 165 , combined by home-prepared (MnCo)Fe 2 O 4 nanozyme-mediated signal amplification. The interfacial photo-induced electron transfer mechanism was chiefly discussed by UV-vis diffuse reflectance spectroscopy in details. Specifically, the (MnCo)Fe 2 O 4 modified VEGF 165 aptamer was released from the PEC aptasensing platform for its highly specific affinity to target VEGF 165 , which terminated the color precipitation reaction, ultimately recovering the PEC signals. The developed sensor displayed a wider linear range from 1 × 10 -2 to 1 × 10 4  pg mL -1 with a smaller limit of detection (LOD) of 0.1 fg mL -1 . This study provides some valuable insights for building other ultrasensitive aptasensors for clinical assays of cancer biomarkers in practice.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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