Fouling of virus filtration membranes by monoclonal antibody feeds with low aggregate content.

Autor: Kaufman Y; TeraPore Technologies, South San Francisco, California, USA., Hunt KC; TeraPore Technologies, South San Francisco, California, USA., Hale G; TeraPore Technologies, South San Francisco, California, USA., McClure M; Department of Chemical Engineering, McMaster University, Hamilton, Ontario, Canada., Latulippe D; Department of Chemical Engineering, McMaster University, Hamilton, Ontario, Canada., Sivan M; TeraPore Technologies, South San Francisco, California, USA., Wilson J; TeraPore Technologies, South San Francisco, California, USA., Dorin R; TeraPore Technologies, South San Francisco, California, USA., Agroskin Y; TeraPore Technologies, South San Francisco, California, USA., Siwak M; TeraPore Technologies, South San Francisco, California, USA., Gerion D; TeraPore Technologies, South San Francisco, California, USA.
Jazyk: angličtina
Zdroj: Biotechnology and bioengineering [Biotechnol Bioeng] 2024 Aug; Vol. 121 (8), pp. 2400-2408. Date of Electronic Publication: 2023 May 10.
DOI: 10.1002/bit.28420
Abstrakt: Membrane fouling by monoclonal antibodies (mAbs) is one of the main challenges in virus-filtration processes. Previous publications attributed membrane fouling to the presence of mAb aggregates in the solution, which block the membrane pores. This fouling mechanism can be solved by a prefilter; however, it was shown that there are mAbs that severely foul the membranes (reduce permeability by 90% and more) even after prefiltering the aggregates, while other mAbs foul the membrane weakly (reduce permeability by ~10% and less). Unfortunately, the differences between the fouling- and the nonfouling mAbs have never been convincingly explained. To get a deeper insight on these differences, we measured the fouling of chemically modified Isoprene-Styrene-4-vinylpyridine (ISV) membranes (TeraPore Technologies) by 8 mAbs exhibiting different hydrophobicity and charge. The results show that mAb solutions with low concentration of aggregates foul ISV membranes via an adsorptive mechanism, and the adsorption is driven mainly by hydrophobic forces between the mAb and the membrane. The charge of the mAbs plays a secondary role in fouling. We want to emphasize that the conclusions pertain to ISV membranes; the insights presented in this paper can potentially be used to engineer new surface chemistries to mitigate fouling of other virus-filtration and/or ultrafiltration membranes.
(© 2023 Wiley Periodicals LLC.)
Databáze: MEDLINE
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