CD3 + T-cell: CD14 + monocyte complexes are dynamic and increased with HIV and glucose intolerance.

Autor:	Obare LM; Division of Infectious Diseases, Vanderbilt University Medical Center, Nashville, TN, USA., Simmons J; Division of Infectious Diseases, Vanderbilt University Medical Center, Nashville, TN, USA., Oakes J; Division of Infectious Diseases, Vanderbilt University Medical Center, Nashville, TN, USA.; Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, Tennessee, USA., Zhang X; Division of Infectious Diseases, Vanderbilt University Medical Center, Nashville, TN, USA., Nochowicz C; Division of Infectious Diseases, Vanderbilt University Medical Center, Nashville, TN, USA., Priest S; Division of Infectious Diseases, Vanderbilt University Medical Center, Nashville, TN, USA., Bailin SS; Division of Infectious Diseases, Vanderbilt University Medical Center, Nashville, TN, USA., Warren CM; Veterans Affairs Tennessee Valley Healthcare System, Nashville, TN, USA., Mashayekhi M; Division of Diabetes, Endocrinology, and Metabolism, Vanderbilt University Medical Center, Nashville, TN, USA., Beasley HK; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, USA., Shao J; Central Microscopy Research Facility, University of Iowa, Iowa City, IA, USA., Meenderink LM; Division of Infectious Diseases, Vanderbilt University Medical Center, Nashville, TN, USA.; Veterans Affairs Tennessee Valley Healthcare System, Nashville, TN, USA., Sheng Q; Department of Biostatistics, Vanderbilt University, Nashville, TN, USA., Stolze J; Department of Biostatistics, Vanderbilt University, Nashville, TN, USA., Gangula R; Division of Infectious Diseases, Vanderbilt University Medical Center, Nashville, TN, USA., Absi T; Department of Cardiac Surgery, Vanderbilt University Medical Center, Nashville, TN, USA., Su YR; Department of Cardiovascular Medicine, Vanderbilt University Medical Center, Nashville, TN, USA., Neikirk K; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, USA., Chopra A; Institute for Immunology and Infectious Diseases, Murdoch University, Murdoch, Western Australia, Australia., Gabriel CL; Division of Gastroenterology, Vanderbilt University Medical Center, Nashville, TN, USA., Temu T; Department of Global Health, University of Washington, Seattle, WA, USA., Pakala S; Division of Infectious Diseases, Vanderbilt University Medical Center, Nashville, TN, USA., Wilfong EM; Division of Rheumatology and Immunology, Vanderbilt University Medical Center, Nashville, TN, USA.; Division of Allergy, Pulmonary, and Critical Care Medicine, Vanderbilt University Medical Center, Nashville, TN, USA., Gianella S; Division of Clinical Pharmacology, Vanderbilt University Medical Center, Nashville, TN, USA., Phillips EJ; Division of Infectious Diseases, Vanderbilt University Medical Center, Nashville, TN, USA.; Institute for Immunology and Infectious Diseases, Murdoch University, Murdoch, Western Australia, Australia.; Division of Allergy, Pulmonary, and Critical Care Medicine, Vanderbilt University Medical Center, Nashville, TN, USA., Harrison DG; Division of Clinical Pharmacology, Vanderbilt University Medical Center, Nashville, TN, USA., Hinton A; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, USA., Kalams SA; Division of Infectious Diseases, Vanderbilt University Medical Center, Nashville, TN, USA.; Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, Tennessee, USA., Kirabo A; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, USA.; Division of Clinical Pharmacology, Vanderbilt University Medical Center, Nashville, TN, USA., Mallal SA; Division of Infectious Diseases, Vanderbilt University Medical Center, Nashville, TN, USA.; Institute for Immunology and Infectious Diseases, Murdoch University, Murdoch, Western Australia, Australia.; Department of Biomedical Informatics, Vanderbilt University, Nashville, TN, USA., Koethe JR; Division of Infectious Diseases, Vanderbilt University Medical Center, Nashville, TN, USA.; Veterans Affairs Tennessee Valley Healthcare System, Nashville, TN, USA., Wanjalla CN; Division of Infectious Diseases, Vanderbilt University Medical Center, Nashville, TN, USA.
Jazyk:	angličtina
Zdroj:	BioRxiv : the preprint server for biology [bioRxiv] 2024 Jun 20. Date of Electronic Publication: 2024 Jun 20.
DOI:	10.1101/2023.04.24.538020
Abstrakt:	An increased risk of cardiometabolic disease accompanies persistent systemic inflammation. Yet, the innate and adaptive immune system features in persons who develop these conditions remain poorly defined. Doublets, or cell-cell complexes, are routinely eliminated from flow cytometric and other immune phenotyping analyses, which limits our understanding of their relationship to disease states. Using well-characterized clinical cohorts, including participants with controlled HIV as a model for chronic inflammation and increased immune cell interactions, we show that circulating CD14 + monocytes complexed to CD3 + T cells are dynamic, biologically relevant, and increased in individuals with diabetes after adjusting for confounding factors. The complexes form functional immune synapses with increased expression of proinflammatory cytokines and greater glucose utilization. Furthermore, in persons with HIV, the CD3 + T-cell: CD14 + monocyte complexes had more HIV copies compared to matched CD14 + monocytes or CD4 + T cells alone. Our results demonstrate that circulating CD3 + T-cell:CD14 + monocyte pairs represent dynamic cellular interactions that may contribute to inflammation and cardiometabolic disease pathogenesis and may originate or be maintained, in part, by chronic viral infections. These findings provide a foundation for future studies investigating mechanisms linking T cellmonocyte cell-cell complexes to developing immune-mediated diseases, including HIV and diabetes. Competing Interests: Declaration of interests The authors have no competing interests.
Databáze:	MEDLINE
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