| Autor: |
Pessoa VA; Instituto Nacional de Pesquisas da Amazônia - INPA, Edible Fungi Cultivation Laboratory, Manaus, AM, Brasil.; Universidade Federal do Amazonas - UFAM, Postgraduate Program in Biotechnology - PPGBIOTEC, Manaus, AM, Brasil., Soares LBN; Instituto Nacional de Pesquisas da Amazônia - INPA, Edible Fungi Cultivation Laboratory, Manaus, AM, Brasil.; Universidade do Estado do Amazonas - UEA, Postgraduate Program in Biodiversity and Biotechnology - PPGBIONORTE, Manaus, AM, Brasil., Silva GL; Instituto Nacional de Pesquisas da Amazônia - INPA, Edible Fungi Cultivation Laboratory, Manaus, AM, Brasil., Vasconcelos AS; Instituto Nacional de Pesquisas da Amazônia - INPA, Edible Fungi Cultivation Laboratory, Manaus, AM, Brasil., Silva JF; Instituto Nacional de Pesquisas da Amazônia - INPA, Edible Fungi Cultivation Laboratory, Manaus, AM, Brasil., Fariña JI; Planta Piloto de Procesos Industriales Microbiológicos - PROIMI-CONICET, San Miguel de Tucumán, Argentina., Oliveira-Junior SD; Instituto Nacional de Pesquisas da Amazônia - INPA, Edible Fungi Cultivation Laboratory, Manaus, AM, Brasil., Sales-Campos C; Instituto Nacional de Pesquisas da Amazônia - INPA, Edible Fungi Cultivation Laboratory, Manaus, AM, Brasil.; Universidade Federal do Amazonas - UFAM, Postgraduate Program in Biotechnology - PPGBIOTEC, Manaus, AM, Brasil.; Universidade do Estado do Amazonas - UEA, Postgraduate Program in Biodiversity and Biotechnology - PPGBIONORTE, Manaus, AM, Brasil., Chevreuil LR; Instituto Nacional de Pesquisas da Amazônia - INPA, Edible Fungi Cultivation Laboratory, Manaus, AM, Brasil. |
| Abstrakt: |
Ganoderma lucidum is a medicinal mushroom widely recognized as a source of biomolecules with pharmacological properties, however, little is known about the factors that influence the synthesis of bioactive proteins by this fungus when cultivated under submerged fermentation. The objective of this work was to evaluate the production of mycelial biomass and intracellular proteases and protease inhibitors by G. lucidum cultivated under different submerged fermentation conditions. The cultivation was carried out in a medium composed of glucose (10 or 20 g.L-1), soy peptone (2.5 or 5 g.L-1) and yeast extract (5 g.L-1), with incubation under agitation (120 rpm) and non-agitation, totaling 8 experimental conditions. Biomass production was determined from the dry weight, while glucose consumption was estimated by quantification of reducing sugars. The proteins were extracted in NaCl (0.15 M), and the protein extracts were submitted to protein quantification by the Bradford method, total proteolytic activity using azocasein, caseinolytic and fibrinolytic activity in Petri dishes, activity of serine (trypsin and chymotrypsin) and cysteine (papain) protease inhibitors. Cultivation in agitated condition showed higher biomass production with a maximum value of 7 g.L-1, in addition to higher activities of trypsin, chymotrypsin and papain inhibitors, with 154 IU.mg-1, 153 IU.mg-1 e 343 IU.mg-1 of protein, respectively. The non-agitated condition showed a greater potential for obtaining proteins, total proteases, caseinolytic and fibrinolytic enzymes, with maximum values of 433 mg.g-1 of extract, 71 U.mL-1 of extract, 63.62 mm2 and 50.27 mm2, respectively. Thus, a medium composed of soy peptone, yest extract and glucose in a 1:2:4 proportion is recommended, under agitation to produce protease inhibitors, and the non-agitated condition when the target is, mainly caseinolytic and fibrinolytic enzymes. |
