| Autor: |
Richardson SS; Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC, USA., Mainville CA; Idexx Laboratories, Westbrook, ME, USA., Arguello-Marin A; Idexx Laboratories, Westbrook, ME, USA., Whalley D; Idexx Laboratories, Westbrook, ME, USA., Burton W; Idexx Laboratories, Westbrook, ME, USA., Breitschwerdt EB; Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC, USA., Qurollo BA; Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC, USA. |
| Abstrakt: |
A validated second-generation SNAP 4Dx Plus (Idexx) incorporates new peptides for improved detection of antibodies against Anaplasma and Ehrlichia tick-borne pathogens in dogs. We compared the first- and second-generation SNAP 4Dx Plus using dogs naturally infected with Anaplasma or Ehrlichia species, or dogs seroreactive by an E. canis indirect fluorescent antibody test (IFAT). The second-generation immunoassay was more sensitive than the first-generation for dogs infected with A. phagocytophilum (51.1% and 29.2%, respectively), A. platys (63.6% and 35.3%, respectively), E. canis (96.2% and 88.3%, respectively), or E. ewingii (73.7% and 70.8%, respectively), and for dogs seroreactive by E. canis IFAT (87.3% and 83.9%, respectively). The second-generation immunoassay detected significantly more Anaplasma - or Ehrlichia -infected dogs that were Anaplasma ( p < 0.001) or Ehrlichia ( p = 0.031) seroreactive, respectively, than did the first-generation test. When Ehrlichia seroreactivity by E. canis IFAT and both immunoassays was compared, significantly more E. canis -infected dogs were seroreactive by E. canis IFAT than the first-generation ( p = 0.006) but not the second-generation ( p = 0.125) immunoassay. Significantly more E. ewingii -infected dogs were seroreactive by the first- ( p = 0.011) and second-generation ( p = 0.049) immunoassays than the E. canis IFAT. Medical records available for 7 dogs that were Anaplasma seroreactive by the second-generation but not the first-generation immunoassay revealed case management decisions that might have been different with an immediate anaplasmosis diagnosis, including earlier doxycycline therapy and less hospitalization. The second-generation SNAP 4Dx Plus test offered improved serologic detection of Anaplasma and Ehrlichia in naturally infected dogs. |
