In-depth Temporal Transcriptome Profiling of Monkeypox and Host Cells using Nanopore Sequencing.

Autor: Kakuk B; Department of Medical Biology, Albert Szent-Györgyi Medical School, University of Szeged, Somogyi u. 4., 6720, Szeged, Hungary., Dörmő Á; Department of Medical Biology, Albert Szent-Györgyi Medical School, University of Szeged, Somogyi u. 4., 6720, Szeged, Hungary., Csabai Z; Department of Medical Biology, Albert Szent-Györgyi Medical School, University of Szeged, Somogyi u. 4., 6720, Szeged, Hungary., Kemenesi G; National Laboratory of Virology, Szentágothai Research Centre, University of Pécs, Pécs, Hungary.; Institute of Biology, Faculty of Sciences, University of Pécs, Pécs, Hungary., Holoubek J; Veterinary Research Institute, Hudcova 70, CZ-62100, Brno, Czech Republic.; Institute of Parasitology, Biology Centre of the Czech Academy of Sciences, Branisovska 31, CZ-37005, Ceske Budejovice, Czech Republic.; Department of Experimental Biology, Faculty of Science, Masaryk University, Kamenice, 753/5, Brno, CZ-62500, Czech Republic., Růžek D; Veterinary Research Institute, Hudcova 70, CZ-62100, Brno, Czech Republic.; Institute of Parasitology, Biology Centre of the Czech Academy of Sciences, Branisovska 31, CZ-37005, Ceske Budejovice, Czech Republic.; Department of Experimental Biology, Faculty of Science, Masaryk University, Kamenice, 753/5, Brno, CZ-62500, Czech Republic., Prazsák I; Department of Medical Biology, Albert Szent-Györgyi Medical School, University of Szeged, Somogyi u. 4., 6720, Szeged, Hungary., Dani VÉ; Department of Medical Biology, Albert Szent-Györgyi Medical School, University of Szeged, Somogyi u. 4., 6720, Szeged, Hungary., Dénes B; Department of Microbiology and Infectious Diseases, University of Veterinary Medicine Budapest, 1143, Budapest, Hungária krt. 23-25, Hungary., Torma G; Department of Medical Biology, Albert Szent-Györgyi Medical School, University of Szeged, Somogyi u. 4., 6720, Szeged, Hungary., Jakab F; National Laboratory of Virology, Szentágothai Research Centre, University of Pécs, Pécs, Hungary.; Institute of Biology, Faculty of Sciences, University of Pécs, Pécs, Hungary., Tóth GE; National Laboratory of Virology, Szentágothai Research Centre, University of Pécs, Pécs, Hungary.; Institute of Biology, Faculty of Sciences, University of Pécs, Pécs, Hungary., Földes FV; National Laboratory of Virology, Szentágothai Research Centre, University of Pécs, Pécs, Hungary.; Institute of Biology, Faculty of Sciences, University of Pécs, Pécs, Hungary., Zana B; National Laboratory of Virology, Szentágothai Research Centre, University of Pécs, Pécs, Hungary.; Institute of Biology, Faculty of Sciences, University of Pécs, Pécs, Hungary., Lanszki Z; National Laboratory of Virology, Szentágothai Research Centre, University of Pécs, Pécs, Hungary.; Institute of Biology, Faculty of Sciences, University of Pécs, Pécs, Hungary., Harangozó Á; Department of Medical Biology, Albert Szent-Györgyi Medical School, University of Szeged, Somogyi u. 4., 6720, Szeged, Hungary., Fülöp Á; Department of Medical Biology, Albert Szent-Györgyi Medical School, University of Szeged, Somogyi u. 4., 6720, Szeged, Hungary., Gulyás G; Department of Medical Biology, Albert Szent-Györgyi Medical School, University of Szeged, Somogyi u. 4., 6720, Szeged, Hungary., Mizik M; Department of Medical Biology, Albert Szent-Györgyi Medical School, University of Szeged, Somogyi u. 4., 6720, Szeged, Hungary., Kiss AA; Department of Medical Biology, Albert Szent-Györgyi Medical School, University of Szeged, Somogyi u. 4., 6720, Szeged, Hungary., Tombácz D; Department of Medical Biology, Albert Szent-Györgyi Medical School, University of Szeged, Somogyi u. 4., 6720, Szeged, Hungary., Boldogkői Z; Department of Medical Biology, Albert Szent-Györgyi Medical School, University of Szeged, Somogyi u. 4., 6720, Szeged, Hungary. boldogkoi.zsolt@med.u-szeged.hu.
Jazyk: angličtina
Zdroj: Scientific data [Sci Data] 2023 May 09; Vol. 10 (1), pp. 262. Date of Electronic Publication: 2023 May 09.
DOI: 10.1038/s41597-023-02149-4
Abstrakt: The recent human Monkeypox outbreak underlined the importance of studying basic biology of orthopoxviruses. However, the transcriptome of its causative agent has not been investigated before neither with short-, nor with long-read sequencing approaches. This Oxford Nanopore long-read RNA-Sequencing dataset fills this gap. It will enable the in-depth characterization of the transcriptomic architecture of the monkeypox virus, and may even make possible to annotate novel host transcripts. Moreover, our direct cDNA and native RNA sequencing reads will allow the estimation of gene expression changes of both the virus and the host cells during the infection. Overall, our study will lead to a deeper understanding of the alterations caused by the viral infection on a transcriptome level.
(© 2023. The Author(s).)
Databáze: MEDLINE
Externí odkaz: