MALDI-TOF as a tool for microbiological monitoring in areas considered aseptic.

Autor: Guimarães ML; Programa de Pós-Graduação Em Microbiologia, Centro Universitário Funorte, Montes Claros, Minas Gerais, Brazil., Júnior MAB; Programa de Pós-Graduação Em Microbiologia, Centro Universitário Funorte, Montes Claros, Minas Gerais, Brazil., de Almeida VM; Instituto de Pesquisas de Produtos Naturais Walter Mors - Universidade Federal Do Rio de Janeiro, Rio de Janeiro, Brazil., Pereira WVS; Setor de Sementes, Departamento de Agricultura - Universidade Federal de Lavras, Lavras, Minas Gerais, Brazil. wvicentesp@gmail.com.
Jazyk: angličtina
Zdroj: Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] [Braz J Microbiol] 2023 Jun; Vol. 54 (2), pp. 761-768. Date of Electronic Publication: 2023 May 09.
DOI: 10.1007/s42770-023-00987-3
Abstrakt: To maintain asepsis in production environments, contamination must be constantly controlled. To this end, microbiological monitoring is constantly used with the objective of evaluating the incidence of microorganisms prevalent in the sampling of air, surface, and people, in the area of an environment considered aseptic, isolated, and identified using the rapid and automated phenotypic microbiological methodology, highlighting the MALDI-TOF mass spectrometry analysis technique (MS), being identified at the level of genus and/or species. For that purpose, microbiological control of environmental monitoring of environments considered aseptic in a pharmaceutical industry was conducted for 12 months. The isolated microorganisms were identified using the mass spectrometry identification method (MALDI-TOF). In area classification A, the most prevalent microorganisms were bacteria in the sampling person. The microbial population was composed of bacteria of the genus Micrococcus sp. and Staphylococcus sp. Based on the results, it is possible to observe that in an environment where the process requires human operations, possible microbial contamination is inevitable and requires the identification of microorganisms at least at the level of species and/or genus. The microorganisms identified and found in the sampling of the aseptic environment must be evaluated with frequency to ensure that the productive environment guarantees the quality of the product produced.
(© 2023. The Author(s) under exclusive licence to Sociedade Brasileira de Microbiologia.)
Databáze: MEDLINE
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