Seedless fruit in Annona squamosa L. is monogenic and conferred by INO locus deletion in multiple accessions.

Autor: Rodrigues BRA; Department of Agricultural Sciences, State University of Montes Claros, Janaúba, MG, 39401-369, Brazil., Gasser CS; Department of Molecular and Cellular Biology, University of California, Davis, Davis, CA, 95616, USA. csgasser@ucdavis.edu., Pimenta S; Department of Agricultural Sciences, State University of Montes Claros, Janaúba, MG, 39401-369, Brazil., Pereira MCT; Department of Agricultural Sciences, State University of Montes Claros, Janaúba, MG, 39401-369, Brazil., Nietsche S; Institute of Agricultural Science, Federal University of Minas Gerais, Montes Claros, Minas Gerais, 39404-547, Brazil. silvia.nietsche@gmail.com.
Jazyk: angličtina
Zdroj: Plant reproduction [Plant Reprod] 2024 Jun; Vol. 37 (2), pp. 71-84. Date of Electronic Publication: 2023 May 09.
DOI: 10.1007/s00497-023-00464-9
Abstrakt: Key Message: Inheritance of the presence/absence of seeds in Annona squamosa is mediated by a single fully recessive gene and is caused by a deletion of the INNER NO OUTER (INO) locus. For some fruits, seedless varieties are desirable for consumption and processing. In the sugar apple tree (Annona squamosa L.), the seedless trait in the Thai seedless (Ts) and Brazilian seedless (Bs) accessions was associated with defective ovules and an apparent deletion of the INNER NO OUTER (INO) ovule development gene locus. Segregation analysis of F 2 and backcross descendants of crosses of Bs to fertile wild-type varieties in this species with a multi-year generation time showed that seedlessness was recessive and controlled by a single locus. Comparison of whole genome sequence of a wild-type plant and a third accession, Hawaiian seedless (Hs), identified a 16 kilobase deletion including INO in this line. Ts and Bs lines were shown to have an identical deletion, indicating a common origin from a single deletion event. Analysis of microsatellite markers could not preclude the possibility that all three seedless accessions are vegetatively propagated clones. The sequence of the deletion site enabled a codominant assay for the wild-type and mutant genes allowing observation of complete cosegregation of the seedless/defective ovule phenotype with the INO deletion, showing maximal separation of less than 3.5 cM. The observed deletion is the only significant difference between the wild-type and Hs line over 587 kilobases, likely encompassing much more than 3.5 cM, showing that the deletion is the cause of seedless trait. The codominant markers and obtained progenies will be useful for introgression of the seedless trait into elite sugar apple lines and into other Annonas through interspecific crossings.
(© 2023. The Author(s).)
Databáze: MEDLINE
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