Genetic models for lineage tracing in musculoskeletal development, injury, and healing.

Autor: Loder S; Department of Plastic Surgery, University of Pittsburgh, Scaife Hall, Suite 6B, 3550 Terrace Street, Pittsburgh, PA 15261, USA., Patel N; Center for Organogenesis and Trauma, Department of Surgery, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA., Morgani S; NYU Langone Orthopedic Hospital, New York, NY, USA., Sambon M; NYU Langone Orthopedic Hospital, New York, NY, USA., Leucht P; NYU Langone Orthopedic Hospital, New York, NY, USA., Levi B; Center for Organogenesis and Trauma, Department of Surgery, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA. Electronic address: benjamin.levi@utsouthwestern.edu.
Jazyk: angličtina
Zdroj: Bone [Bone] 2023 Aug; Vol. 173, pp. 116777. Date of Electronic Publication: 2023 May 06.
DOI: 10.1016/j.bone.2023.116777
Abstrakt: Musculoskeletal development and later post-natal homeostasis are highly dynamic processes, marked by rapid structural and functional changes across very short periods of time. Adult anatomy and physiology are derived from pre-existing cellular and biochemical states. Consequently, these early developmental states guide and predict the future of the system as a whole. Tools have been developed to mark, trace, and follow specific cells and their progeny either from one developmental state to the next or between circumstances of health and disease. There are now many such technologies alongside a library of molecular markers which may be utilized in conjunction to allow for precise development of unique cell 'lineages'. In this review, we first describe the development of the musculoskeletal system beginning as an embryonic germ layer and at each of the key developmental stages that follow. We then discuss these structures in the context of adult tissues during homeostasis, injury, and repair. Special focus is given in each of these sections to the key genes involved which may serve as markers of lineage or later in post-natal tissues. We then finish with a technical assessment of lineage tracing and the techniques and technologies currently used to mark cells, tissues, and structures within the musculoskeletal system.
Competing Interests: Declaration of competing interest No competing interests to declare.
(Copyright © 2023. Published by Elsevier Inc.)
Databáze: MEDLINE
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