A tessellated lymphoid network provides whole-body T cell surveillance in zebrafish.

Autor:	Robertson TF; Department of Medical Microbiology and Immunology, University of Wisconsin-Madison, Madison, WI 53706., Hou Y; Department of Medical Microbiology and Immunology, University of Wisconsin-Madison, Madison, WI 53706., Schrope J; Department of Biomedical Engineering, University of Wisconsin-Madison, Madison, WI 53726., Shen S; Department of Medical Microbiology and Immunology, University of Wisconsin-Madison, Madison, WI 53706., Rindy J; Department of Medical Microbiology and Immunology, University of Wisconsin-Madison, Madison, WI 53706., Sauer JD; Department of Medical Microbiology and Immunology, University of Wisconsin-Madison, Madison, WI 53706., Dinh HQ; McArdle Laboratory for Cancer Research, University of Wisconsin-Madison, Madison, WI 53705., Huttenlocher A; Department of Medical Microbiology and Immunology, University of Wisconsin-Madison, Madison, WI 53706.; Department of Pediatrics, University of Wisconsin-Madison, Madison, WI 53792.
Jazyk:	angličtina
Zdroj:	Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2023 May 16; Vol. 120 (20), pp. e2301137120. Date of Electronic Publication: 2023 May 08.
DOI:	10.1073/pnas.2301137120
Abstrakt:	Homeostatic trafficking to lymph nodes allows T cells to efficiently survey the host for cognate antigen. Nonmammalian jawed vertebrates lack lymph nodes but maintain diverse T cell pools. Here, we exploit in vivo imaging of transparent zebrafish to investigate how T cells organize and survey for antigen in an animal devoid of lymph nodes. We find that naïve-like T cells in zebrafish organize into a previously undescribed whole-body lymphoid network that supports streaming migration and coordinated trafficking through the host. This network has the cellular hallmarks of a mammalian lymph node, including naïve T cells and CCR7-ligand expressing nonhematopoietic cells, and facilitates rapid collective migration. During infection, T cells transition to a random walk that supports antigen-presenting cell interactions and subsequent activation. Our results reveal that T cells can toggle between collective migration and individual random walks to prioritize either large-scale trafficking or antigen search in situ. This lymphoid network thus facilitates whole-body T cell trafficking and antigen surveillance in the absence of a lymph node system.
Databáze:	MEDLINE
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