Effects of leptin on the viability of human ovarian cancer cells and changes in cytokine expression levels.

Autor:	Dincer F; Divison of Gynecologic Oncology, Health Sciences University İzmir Tepecik Education and Research Hospital, Health Sciences University, Izmir, Turkey., Atmaca H; Department of Biology, Faculty of Science and Letters, Manisa Celal Bayar University, Manisa, Turkey., Akman L; Department of Obstetrics and Gynecology, Divison of Gynecologic Oncology, Ege University, Bayraklı, İzmir, Turkey., Oktay LM; Department of Medical Biology, Medicine Faculty, Ege University, Bayraklı, İzmir, Turkey., Karaca B; Department of Medical Oncology, Tulay Aktas Oncology Hospital, Ege University, Bayraklı, İzmir, Turkey., Terek MC; Department of Obstetrics and Gynecology, Divison of Gynecologic Oncology, Ege University, Bayraklı, İzmir, Turkey.
Jazyk:	angličtina
Zdroj:	PeerJ [PeerJ] 2023 Apr 20; Vol. 11, pp. e15246. Date of Electronic Publication: 2023 Apr 20 (Print Publication: 2023).
DOI:	10.7717/peerj.15246
Abstrakt:	Background: Obesity is associated with increased mortality among ovarian cancer and is a poor prognostic factor. There are significant links between the leptin hormone, a product of the obesity gene, and the development of ovarian cancer. Leptin is a vital hormone-like cytokine secreted from adipose tissue and is mainly involved in the maintenance of energy homeostasis. It regulates several intracellular signaling pathways and also interacts with various hormones and energy regulators. It acts as a growth factor by stimulating cell proliferation and differentiation and in this way contributes to cancer cell development. The aim of the study was to investigate the effects of leptin on human ovarian cancer cells. Methods: In this study, the effects of increasing the concentration of leptin were investigated on the cell viability of OVCAR-3 and MDAH-2774 ovarian cancer lines by MTT assay. Moreover, to elucidate the molecular mechanisms of leptin in ovarian cancer cells, changes in the expression levels of 80 cytokines were evaluated after leptin treatment via a human cytokine antibody array. Results: Leptin increases the proliferation of both ovarian cancer cell lines. IL-1 level was increased in OVCAR-3 cells and TGF-β level was increased in MDAH-2774 cells after leptin treatment. A decrease in IL-2, MCP-2/CCL8 and MCP-3/CCL7 levels was detected in both ovarian cancer cell lines with leptin administration. An increase in IL-3 and IL-10 expressions, insulin-like growth factor binding proteins (IGFBP) IGFBP-1, IGFBP-2 and IGFBP-3 levels were detected in both ovarian cancer cell lines with leptin administration. In conclusion; leptin has a proliferative effect on human ovarian cancer cell lines and affects different cytokines in different types of ovarian cancer cells. Competing Interests: The authors declare that they have no competing interests. (© 2023 Dincer et al.)
Databáze:	MEDLINE
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