Fluorescence turn-on detection of human serum albumin based on the assembly of gold nanoclusters and bromocresol green.

Autor: Xiao W; School of Life and Environmental Sciences, Guilin University of Electronic Technology, Guilin, 541004, China. wenxiangxiao@126.com.; Guangxi Colleges and Universities Key Laboratory of Biomedical Sensing and Intelligent Instrument, Guilin University of Electronic Technology, Guilin, 541004, China. wenxiangxiao@126.com., Li Y; School of Life and Environmental Sciences, Guilin University of Electronic Technology, Guilin, 541004, China., Xiong Y; School of Life and Environmental Sciences, Guilin University of Electronic Technology, Guilin, 541004, China., Chen Z; Guangxi Colleges and Universities Key Laboratory of Biomedical Sensing and Intelligent Instrument, Guilin University of Electronic Technology, Guilin, 541004, China., Li H; School of Life and Environmental Sciences, Guilin University of Electronic Technology, Guilin, 541004, China. lihua@guet.edu.cn.; Guangxi Colleges and Universities Key Laboratory of Biomedical Sensing and Intelligent Instrument, Guilin University of Electronic Technology, Guilin, 541004, China. lihua@guet.edu.cn.
Jazyk: angličtina
Zdroj: Analytical and bioanalytical chemistry [Anal Bioanal Chem] 2023 Jul; Vol. 415 (17), pp. 3363-3374. Date of Electronic Publication: 2023 May 08.
DOI: 10.1007/s00216-023-04717-4
Abstrakt: As the most abundant protein in plasma, human serum albumin plays a vital role in physiological processes, such as maintaining blood osmotic pressure and carrying small-molecule ligands. Since the content of albumin in the human serum can reflect the status of liver and renal function, albumin quantitation is significant in clinical diagnosis. In this work, fluorescence turn-on detection of human serum albumin (HSA) had been performed based on the assembly of gold nanoclusters and bromocresol green. Gold nanoclusters (AuNCs) capped by reduced glutathione (GSH) were assembled with bromocresol green (BCG), and the assembly was used as a fluorescent probe for HSA. After BCG assembling, the fluorescence of gold nanoclusters was nearly quenched. In acidic solution, HSA can selectively bind to BCG on the assembly and recover the fluorescence of the solution. Based on this turn-on fluorescence, ratiometric HSA quantification was realized. Under optimal conditions, HSA detection by the probe possessed a good linear relationship in the range of 0.40-22.50 mg·mL -1 , and the detection limit was 0.27 ± 0.04 mg·mL -1 (3σ, n = 3). Common coexisting components in serum and blood proteins did not interfere with the detection of HSA. This method has the advantages of easy manipulation and high sensitivity, and the fluorescent response is insensitive to reaction time.
(© 2023. Springer-Verlag GmbH Germany, part of Springer Nature.)
Databáze: MEDLINE
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