NF-κB signaling pathway mechanism in cow intertoe skin inflammation caused by Fusobacterium necrophorum .

Autor:	Zhang H; College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, China., Zheng J; College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, China., Sun Y; College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, China., Yang C; College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, China., Yue Y; College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, China.
Jazyk:	angličtina
Zdroj:	Frontiers in cellular and infection microbiology [Front Cell Infect Microbiol] 2023 Apr 21; Vol. 13, pp. 1156449. Date of Electronic Publication: 2023 Apr 21 (Print Publication: 2023).
DOI:	10.3389/fcimb.2023.1156449
Abstrakt:	Background: Fusobacterium necrophorum is the main pathogen inducing bovine foot rot. The infected site is often accompanied by a strong inflammatory response, but the specific inflammatory regulatory mechanism remains unclear. Aim: A cow skin explants model was established to elucidate the mechanism of F. necrophorum bacillus causing foot rot in cows, and to provide reference for future clinical practice. Methods: Cow intertoe skin explants were cultured in vitro , and F. necrophorum bacteria solution and nuclear factor-κB (NF-κB) inhibitor BAY 1-7082 were added to establish an in vitro infection model. Hematoxylin and eosin staining, terminal - deoxynucleotidyl transferase mediated nick end labeling, and immunohistochemistry were used to detect the pathological changes of the skin explants infected with F. necrophorum , the degree of tissue cell apoptosis, and the expression of the apoptosis-related protein Caspase-3, respectively. RT-qPCR, Western blot, and ELISA were used to detect the activation of the NF-κB pathway and inflammatory cytokines by F. necrophorum . Results: The intertoe skin structure of cows infected with F. necrophorum changed with different degrees of inflammation, and the degree of tissue cell apoptosis was significantly increased ( P < 0.01). In addition, infection with F. necrophorum significantly increased the phosphorylation level of IκBα protein and up-regulated the expression level of NF-κB p65. The high expression and transcriptional activity of NF-κB p65 significantly increased the expression and concentration of the inflammatory cytokines TNF-α, IL-1β, and IL-8, thus inducing the occurrence of an inflammatory response. However, inhibition of NF-κB p65 activity significantly decreased the expression of inflammatory factors in the intertoe skin of cows infected with F. necrophorum . Conclusion: F. necrophorum activates NF-κB signaling pathway by increasing the expression of TNF-α, IL-1β, IL-8 and other inflammatory factors, leading to foot rot in dairy cows. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2023 Zhang, Zheng, Sun, Yang and Yue.)
Databáze:	MEDLINE
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