Gold nanoparticle intratesticular injections as a potential animal sterilization tool: Long-term reproductive and toxicological implications.

Autor: Coimbra JLP; Laboratory of Cellular Biology, Department of Morphology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil., Dantas GPF; Laboratory of Cellular Biology, Department of Morphology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil., de Andrade LM; Laboratory of Nanomaterials, ICEX/UFMG, Nanobiomedical Research Group, Belo Horizonte, MG, Brazil., Brener MRG; Laboratory of Cellular Biology, Department of Morphology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil., Viana PIM; Laboratory of Cellular Biology, Department of Morphology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil., Lopes RA; Laboratory of Endocrinology and Metabolism, Department of Physiology and Pharmacology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil., O G Gontijo D; Department of Physiology and Biophysics, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil., Ervilha LOG; Laboratory of Animal Reproduction and Toxicology, Department of General Biology, Viçosa, MG, Brazil., Assis MQ; Laboratory of Animal Reproduction and Toxicology, Department of General Biology, Viçosa, MG, Brazil., Barcelos LS; Department of Physiology and Biophysics, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil., E Szawka R; Laboratory of Endocrinology and Metabolism, Department of Physiology and Pharmacology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil., Damasceno DC; Laboratory of Experimental Research on Gynecology and Obstetrics, Postgraduate Course of Tocogynecology, Botucatu Medical School, Unesp, Brazil., Machado-Neves M; Laboratory of Animal Reproduction and Toxicology, Department of General Biology, Viçosa, MG, Brazil., Mota AP; Clinical Hematology Laboratory, Faculty of Pharmacy, Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil., Costa GMJ; Laboratory of Cellular Biology, Department of Morphology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil. Electronic address: gmjc@ufmg.br.
Jazyk: angličtina
Zdroj: Toxicology [Toxicology] 2023 Jun 15; Vol. 492, pp. 153543. Date of Electronic Publication: 2023 May 05.
DOI: 10.1016/j.tox.2023.153543
Abstrakt: This study aimed to evaluate the gold nanoparticles (AuNPs) animal sterilizing potential after intratesticular injections and long-term adverse reproductive and systemic effects. Adult male Wistar rats were divided into control and gold nanoparticle (AuNPs) groups. The rats received 200 µL of saline or AuNPs solution (16 µg/mL) on experimental days 1 and 7 (ED1 and ED7). After 150 days, the testicular blood flow was measured, and the rats were mated with females. After mating, male animals were euthanized for histological, cellular, and molecular evaluations. The female fertility indices and fetal development were also recorded. The results indicated increased blood flow in the testes of treated animals. Testes from treated rats had histological abnormalities, shorter seminiferous epithelia, and oxidative stress. Although the sperm concentration was lower in the AuNP-treated rats, there were no alterations in sperm morphology. Animals exposed to AuNPs had decreased male fertility indices, and their offspring had lighter and less efficient placentas. Additionally, the anogenital distance was longer in female fetuses. There were no changes in the histology of the kidney and liver, the lipid profile, and the serum levels of LH, testosterone, AST, ALT, ALP, albumin, and creatinine. The primary systemic effect was an increase in MDA levels in the liver and kidney, with only the liver experiencing an increase in CAT activity. In conclusion, AuNPs have a long-term impact on reproduction with very slight alterations in animal health. The development of reproductive biotechnologies that eliminate germ cells or treat local cancers can benefit from using AuNPs.
Competing Interests: Declaration of Competing Interest The authors declare that they have no competing interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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