Effect of the Fetal THRB Genotype on the Placenta.

Autor:	Salas-Lucia F; Departments of Medicine, University of Chicago, Chicago, IL 60637, USA., Stan MN; Division of Endocrinology, Mayo Clinic, Rochester, MN 55905, USA., James H; Division of Endocrinology, Mayo Clinic, Rochester, MN 55905, USA., Rajwani A; Division of Endocrinology, Mayo Clinic, Rochester, MN 55905, USA., Liao XH; Departments of Medicine, University of Chicago, Chicago, IL 60637, USA., Dumitrescu AM; Departments of Medicine, University of Chicago, Chicago, IL 60637, USA.; Departments of Molecular Metabolism and Nutrition, University of Chicago, Chicago, IL 60637, USA., Refetoff S; Departments of Medicine, University of Chicago, Chicago, IL 60637, USA.; Departments of Pediatrics, University of Chicago, Chicago, IL 60637.; Departments of Committees on Genetics, University of Chicago, Chicago, IL 60637, USA.
Jazyk:	angličtina
Zdroj:	The Journal of clinical endocrinology and metabolism [J Clin Endocrinol Metab] 2023 Sep 18; Vol. 108 (10), pp. e944-e948.
DOI:	10.1210/clinem/dgad243
Abstrakt:	Context: Pregnant women with mutations in the thyroid hormone receptor beta (THRB) gene expose their fetuses to high thyroid hormone (TH) levels shown to be detrimental to a normal fetus (NlFe) but not to an affected fetus (AfFe). However, no information is available about differences in placental TH regulators. Objective: To investigate whether there are differences in placentas associated with a NlFe compared with an AfFe, we had the unique opportunity to study placentas from 2 pregnancies of the same woman with THRB mutation G307D. One placenta supported a NlFe while the other an AfFe. Methods: Sections of placentas were collected and frozen at -80 °C after term delivery of a NlFe and an AfFe. Two placentas from healthy women of similar gestational age were also obtained. The fetal origin of the placental tissues was established by gDNA quantitation of genes on the X and Y chromosomes and THRB gene. Expression and enzymatic activity of deiodinases 2 and 3 were measured. Expression of following genes was also quantitated: MCT10, MCT8, LAT1, LAT2, THRB, THRA. Results: The placenta carrying the AfFe exhibited a significant reduction of deiodinase 2 and 3 activities as well as the expression of the TH transporters MCT10, LAT1 and LAT2, and THRA. Conclusion: We present the first study of the effect of the fetal THRB genotype on the placenta. Though limited by virtue of the rarity of THRB mutations and sample availability, we show that the fetal THRB genotype influences the levels of TH regulators in the placenta. (© The Author(s) 2023. Published by Oxford University Press on behalf of the Endocrine Society. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)
Databáze:	MEDLINE
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