Long-Read Metagenomics and CAZyme Discovery.
| Autor: | Ferrillo A; Faculty of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, Aas, Norway., Kobel CM; Faculty of Bioscience, Norwegian University of Life Sciences, Aas, Norway., Vera-Ponce de León A; Faculty of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, Aas, Norway.; Faculty of Bioscience, Norwegian University of Life Sciences, Aas, Norway., La Rosa SL; Faculty of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, Aas, Norway., Kunath BJ; Luxembourg Centre for Systems Biomedicine, Esch-sur-Alzette, Luxembourg., Pope PB; Faculty of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, Aas, Norway.; Faculty of Bioscience, Norwegian University of Life Sciences, Aas, Norway., Hagen LH; Faculty of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, Aas, Norway. live.hagen@nmbu.no. |
|---|---|
| Jazyk: | angličtina |
| Zdroj: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2023; Vol. 2657, pp. 253-284. |
| DOI: | 10.1007/978-1-0716-3151-5_19 |
| Abstrakt: | Microorganisms play a primary role in regulating biogeochemical cycles and are a valuable source of enzymes that have biotechnological applications, such as carbohydrate-active enzymes (CAZymes). However, the inability to culture the majority of microorganisms that exist in natural ecosystems restricts access to potentially novel bacteria and beneficial CAZymes. While commonplace molecular-based culture-independent methods such as metagenomics enable researchers to study microbial communities directly from environmental samples, recent progress in long-read sequencing technologies are advancing the field. We outline key methodological stages that are required as well as describe specific protocols that are currently used for long-read metagenomic projects dedicated to CAZyme discovery. (© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.) |
| Databáze: | MEDLINE |
| Externí odkaz: |
|