Efficacy of electrical pulse mediated tomato lipophilic extract on human breast cancer cell.

Autor: Thulasidas JS; Department of sEEE, College of Engineering, Division of High Voltage Engineering, Anna University, Chennai, Tamil Nadu, India., Varadarajan GS; Department of sEEE, College of Engineering, Division of High Voltage Engineering, Anna University, Chennai, Tamil Nadu, India., Camarillo I; Department of Biological Sciences; Center for Cancer Research, Purdue University, West Lafayette, Indiana, USA, India., Mittal L; School of Engineering Technology, Purdue University, West Lafayette, Indiana, USA., Sundararajan R; School of Engineering Technology, Purdue University, West Lafayette, Indiana, USA.
Jazyk: angličtina
Zdroj: Journal of cancer research and therapeutics [J Cancer Res Ther] 2023 Apr; Vol. 19 (Supplement), pp. S106-S115.
DOI: 10.4103/jcrt.JCRT_1117_19
Abstrakt: Aim: The purpose of this research is to study the effect of electrical pulse mediated tomato lipophilic extract (TLE) on human breast cancer MCF-7 and non-tumorigenic MCF-10A cells.
Materials and Methods: MCF-7 and MCF-10A cells were treated with 50 μg/mL TLE and eight 100 μs electric pulses of different electric field intensities (800, 1000, and 1200 V/cm), and the viability was studied using real time MT assay at 24 h of treatment. In addition, we studied cell viability of both the cells at 0 h using trypan blue assay and the ability to form colonies of both cells using colony forming unit (CFU) assay for all the treatments. We also imaged the cells at 24 h using microscope.
Results: With 50 μg/mL TLE, the cell viability of MCF-7 and MCF-10A was same (84%). When the same concentration of TLE is combined with eight electrical pulses of 1200 V/cm, the cell viability of MCF-7 and MCF-10A was 2% and 87%, respectively. These results indicate that the effect of electrical pulses mediated TLE was higher on cancerous MCF-7 cells when compared to non-cancerous MCF-10A cells.
Conclusion: The combination of electrical pulses with TLE is an effective strategy to selectively target cancer cells in the body.
Competing Interests: None
Databáze: MEDLINE
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