Analysis of Defense-Related Gene Expression and Leaf Metabolome in Wheat During the Early Infection Stages of Blumeria graminis f. sp. tritici .

Autor:	Allario T; Univ. Littoral Côte d'Opale, Unité de Chimie Environnementale et Interactions sur le Vivant (UCEIV-UR 4492), SFR Condorcet FR CNRS 3417, CS 80699, F-62228, Calais cedex, France., Fourquez A; Univ. Littoral Côte d'Opale, Unité de Chimie Environnementale et Interactions sur le Vivant (UCEIV-UR 4492), SFR Condorcet FR CNRS 3417, CS 80699, F-62228, Calais cedex, France., Magnin-Robert M; Univ. Littoral Côte d'Opale, Unité de Chimie Environnementale et Interactions sur le Vivant (UCEIV-UR 4492), SFR Condorcet FR CNRS 3417, CS 80699, F-62228, Calais cedex, France., Siah A; Joint Research Unit 1158 BioEcoAgro, Junia, Univ. Lille, Univ. Liège, UPJV, ULCO, Univ. Artois, INRAE, 2 Rue Norbert Ségard, F-59014, Lille, France., Maia-Grondard A; Université de Strasbourg, INRAE, SVQV UMR-A 1131, F-68000 Colmar, France., Gaucher M; IRHS-UMR1345, Université d'Angers, INRAE, Institut Agro, SFR 4207 QuaSaV, F-49071, F-49071 Beaucouzé cedex, France., Brisset MN; IRHS-UMR1345, Université d'Angers, INRAE, Institut Agro, SFR 4207 QuaSaV, F-49071, F-49071 Beaucouzé cedex, France., Hugueney P; Université de Strasbourg, INRAE, SVQV UMR-A 1131, F-68000 Colmar, France., Reignault P; Univ. Littoral Côte d'Opale, Unité de Chimie Environnementale et Interactions sur le Vivant (UCEIV-UR 4492), SFR Condorcet FR CNRS 3417, CS 80699, F-62228, Calais cedex, France., Baltenweck R; Université de Strasbourg, INRAE, SVQV UMR-A 1131, F-68000 Colmar, France., Randoux B; Univ. Littoral Côte d'Opale, Unité de Chimie Environnementale et Interactions sur le Vivant (UCEIV-UR 4492), SFR Condorcet FR CNRS 3417, CS 80699, F-62228, Calais cedex, France.
Jazyk:	angličtina
Zdroj:	Phytopathology [Phytopathology] 2023 Aug; Vol. 113 (8), pp. 1537-1547. Date of Electronic Publication: 2023 Sep 29.
DOI:	10.1094/PHYTO-10-22-0364-R
Abstrakt:	Blumeria graminis f. sp. tritici ( Bgt ) is an obligate biotrophic fungal pathogen responsible for powdery mildew in bread wheat ( Triticum aestivum ). Upon Bgt infection, the wheat plant activates basal defense mechanisms, namely PAMP-triggered immunity, in the leaves during the first few days. Understanding this early stage of quantitative resistance is crucial for developing new breeding tools and evaluating plant resistance inducers for sustainable agricultural practices. In this sense, we used a combination of transcriptomic and metabolomic approaches to analyze the early steps of the interaction between Bgt and the moderately susceptible wheat cultivar Pakito. Bgt infection resulted in an increasing expression of genes encoding pathogenesis-related (PR) proteins ( PR1 , PR4 , PR5 , and PR8 ) known to target the pathogen, during the first 48 h postinoculation. Moreover, RT-qPCR and metabolomic analyses pointed out the importance of the phenylpropanoid pathway in quantitative resistance against Bgt . Among metabolites linked to this pathway, hydroxycinnamic acid amides containing agmatine and putrescine as amine components accumulated from the second to the fourth day after inoculation. This suggests their involvement in quantitative resistance via cross-linking processes in cell walls for reinforcement, which is supported by the up-regulation of PAL (phenylalanine ammonia-lyase), PR15 (oxalate oxidase) and POX (peroxidase) after inoculation. Finally, pipecolic acid, which is considered a signal involved in systemic acquired resistance, accumulated after inoculation. These new insights lead to a better understanding of basal defense in wheat leaves after Bgt infection. Competing Interests: The author(s) declare no conflict of interest.
Databáze:	MEDLINE
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