Refrigerated modified Knott concentrate enables long-term morphological viability of canine blood microfilariae.

Autor: Zanfagnini LG; Veterinarian, MSc., Programa de Pós-Graduação em Sanidade e Produção Animal Sustentável na Amazônia (PPGESPA), Centro de Ciências Biológicas e da Natureza, Universidade Federal do Acre (UFAC). Rio Branco, AC, Brazil., da Silva TP; Veterinarian, Autonomous, Muriaé, MG, Brazil., Campos DR; Veterinarian, DSc. Programa de Pós-Graduação em Ciências Veterinárias (PPGCV), Departamento de Parasitologia Animal (DPA). Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro. Seropédica, RJ. Brazil., de Souza SF; Veterinarian, DSc., Centro de Ciências Biológicas e da Natureza, UFAC. Rio Branco, AC, Brazil., Malavazi PFNDS; Veterinarian, DSc., Centro de Ciências Biológicas e da Natureza, UFAC. Rio Branco, AC, Brazil., de Oliveira RS; Veterinarian, DSc., Centro de Ciências Biológicas e da Natureza, UFAC. Rio Branco, AC, Brazil., Daudt C; Veterinarian, DSc., Centro de Ciências Biológicas e da Natureza, UFAC. Rio Branco, AC, Brazil., Pacheco AD; Veterinarian, DSc., Centro de Ciências Biológicas e da Natureza, UFAC. Rio Branco, AC, Brazil.
Jazyk: angličtina
Zdroj: Brazilian journal of veterinary medicine [Braz J Vet Med] 2023 Apr 25; Vol. 45, pp. e000223. Date of Electronic Publication: 2023 Apr 25 (Print Publication: 2023).
DOI: 10.29374/2527-2179.bjvm000223
Abstrakt: There are several methods of laboratory diagnosis of filarids, the most used are the thick smear and the Knott method. Both are quick to perform, have a low cost and allow observing the presence, quantifying and analyzing the morphological characteristics of microfilariae. Knowing the morphological viability of fixed microfilariae is of practical importance, as it allows the transport of samples to a laboratory, facilitates epidemiological studies , and allows the storage of samples for didactic. Thus, the aim of this study was to evaluate the morphological viability of microfilariae fixed in the refrigerated modified knott test using 2% formalin. To perform the modified Knott technique, 10 samples of microfilaremic dogs aged over 6 months were used. To evaluate the morphological viability time of the microfilariae in the modified Knott concentrate, the evaluations were repeated after intervals of 0, 1, 7, 30, 60, 120, 180, 240, and 304 days. In the present study, we did not verify any difference in the morphology of the microfilariae in any of the analyzed intervals from day 0 to 304 days, and it is possible to conclude that the use of 2% formalin in the modified Knott technique allows the microfilariae to be identified in a period of 304 days. days after processing the sample without changes in its morphology.
Competing Interests: Conflicts of interests: The authors declare that they have no competing interest.
Databáze: MEDLINE