Catechin potentiates the antifungal effect of miconazole in Candida glabrata.

Autor: Hervay NT; Faculty of Natural Sciences, Department of Microbiology and Virology, Comenius University in Bratislava, Ilkovicova 6, Bratislava, 842 15, Slovak Republic., Elias D; Faculty of Natural Sciences, Department of Microbiology and Virology, Comenius University in Bratislava, Ilkovicova 6, Bratislava, 842 15, Slovak Republic., Habova M; Faculty of Natural Sciences, Department of Microbiology and Virology, Comenius University in Bratislava, Ilkovicova 6, Bratislava, 842 15, Slovak Republic., Jacko J; Faculty of Mathematics, Physics and Informatics, Department of Nuclear Physics and Biophysics, Comenius University in Bratislava, Mlynska Dolina, Bratislava, 842 48, Slovak Republic., Morvova M Jr; Faculty of Mathematics, Physics and Informatics, Department of Nuclear Physics and Biophysics, Comenius University in Bratislava, Mlynska Dolina, Bratislava, 842 48, Slovak Republic., Gbelska Y; Faculty of Natural Sciences, Department of Microbiology and Virology, Comenius University in Bratislava, Ilkovicova 6, Bratislava, 842 15, Slovak Republic. yvetta.gbelska@uniba.sk.
Jazyk: angličtina
Zdroj: Folia microbiologica [Folia Microbiol (Praha)] 2023 Dec; Vol. 68 (6), pp. 835-842. Date of Electronic Publication: 2023 May 05.
DOI: 10.1007/s12223-023-01061-z
Abstrakt: The rising number of invasive fungal infections caused by drug-resistant Candida strains is one of the greatest challenges for the development of novel antifungal strategies. The scarcity of available antifungals has drawn attention to the potential of natural products as antifungals and in combinational therapies. One of these is catechins-polyphenolic compounds-flavanols, found in a variety of plants. In this work, we evaluated the changes in the susceptibility of Candida glabrata strain characterized at the laboratory level and clinical isolates using the combination of catechin and antifungal azoles. Catechin alone had no antifungal activity within the concentration range tested. Its use in combination with miconazole resulted in complete inhibition of growth in the sensitive C. glabrata isolate and a significant growth reduction in the azole resistant C. glabrata clinical isolate. Simultaneous use of catechin and miconazole leads to increased intracellular ROS generation. The enhanced susceptibility of C. glabrata clinical isolates to miconazole by catechin was accompanied with the intracellular accumulation of ROS and changes in the plasma membrane permeability, as measured using fluorescence anisotropy, affecting the function of plasma membrane proteins.
(© 2023. The Author(s).)
Databáze: MEDLINE
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