Transwell In Vitro Cell Migration and Invasion Assays.

Autor:	Justus CR; Department of Internal Medicine, Brody School of Medicine, East Carolina University, Greenville, NC, USA., Marie MA; Department of Internal Medicine, Brody School of Medicine, East Carolina University, Greenville, NC, USA., Sanderlin EJ; Department of Internal Medicine, Brody School of Medicine, East Carolina University, Greenville, NC, USA., Yang LV; Department of Internal Medicine, Brody School of Medicine, East Carolina University, Greenville, NC, USA. yangl@ecu.edu.
Jazyk:	angličtina
Zdroj:	Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2023; Vol. 2644, pp. 349-359.
DOI:	10.1007/978-1-0716-3052-5_22
Abstrakt:	Cell migration and invasion have essential roles in both normal physiology and disease. As such, methodologies to assess cell migratory and invasive capacities are necessary to elucidate normal cell processes and underlying mechanisms of disease. Here, we describe commonly used transwell in vitro methods for the study of cell migration and invasion. The transwell migration assay involves the chemotaxis of cells through a porous membrane after the establishment of a chemoattractant gradient using two medium-filled compartments. The transwell invasion assay involves the addition of an extracellular matrix on top of the porous membrane which only permits chemotaxis of cells which possess invasive properties such as tumor cells. (© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze:	MEDLINE
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