Construction and evaluation of intrauterine adhesion model in rats by different methods of mechanical injury.

Autor: Chen F; Medical Center of Diagnosis and Treatment for Cervical Diseases, Obstetrics and Gynecology Hospital, Fudan University, 419 Fangxie Road, Shanghai, 200011, China.; Shanghai Key Laboratory of Female Reproductive Endocrine Related Diseases, Shanghai, 200011, China., Gong YX; Medical Center of Diagnosis and Treatment for Cervical Diseases, Obstetrics and Gynecology Hospital, Fudan University, 419 Fangxie Road, Shanghai, 200011, China.; Shanghai Key Laboratory of Female Reproductive Endocrine Related Diseases, Shanghai, 200011, China., Xiao JJ; Medical Center of Diagnosis and Treatment for Cervical Diseases, Obstetrics and Gynecology Hospital, Fudan University, 419 Fangxie Road, Shanghai, 200011, China.; Shanghai Key Laboratory of Female Reproductive Endocrine Related Diseases, Shanghai, 200011, China., Jiang NH; Medical Center of Diagnosis and Treatment for Cervical Diseases, Obstetrics and Gynecology Hospital, Fudan University, 419 Fangxie Road, Shanghai, 200011, China.; Shanghai Key Laboratory of Female Reproductive Endocrine Related Diseases, Shanghai, 200011, China., Chen LM; Medical Center of Diagnosis and Treatment for Cervical Diseases, Obstetrics and Gynecology Hospital, Fudan University, 419 Fangxie Road, Shanghai, 200011, China. limei1118@foxmail.com.; Shanghai Key Laboratory of Female Reproductive Endocrine Related Diseases, Shanghai, 200011, China. limei1118@foxmail.com., Sui L; Medical Center of Diagnosis and Treatment for Cervical Diseases, Obstetrics and Gynecology Hospital, Fudan University, 419 Fangxie Road, Shanghai, 200011, China. suilong@fudan.edu.cn.; Shanghai Key Laboratory of Female Reproductive Endocrine Related Diseases, Shanghai, 200011, China. suilong@fudan.edu.cn.
Jazyk: angličtina
Zdroj: Archives of gynecology and obstetrics [Arch Gynecol Obstet] 2023 Jul; Vol. 308 (1), pp. 281-290. Date of Electronic Publication: 2023 May 05.
DOI: 10.1007/s00404-023-06926-9
Abstrakt: Purpose: The study aimed to establish a stable and effective animal model for the experimental study of intrauterine adhesion (IUA) by evaluating various mechanical injury methods.
Methods: A total of 140 female rats were divided into four groups according to the extent and area of endometrial injury: group A (excision area: 2.0 × 0.5 cm 2 ), group B (excision area: 2.0 × 0.25 cm 2 ), group C (endometrial curettage) and group D (sham operation). On the 3rd, 7th, 15th and 30th day after the operation, the tissue samples of each group were collected, and the uterine cavity stenosis and histological changes were recorded by HE and Masson staining. Immunohistochemistry of CD31 was applied to visualize microvessel density (MVD). The pregnancy rate and the number of gestational sacs were used to evaluate the reproductive outcome.
Results: The results showed that endometrium injured by small-area endometrial excision or simple curettage could be repaired. The ratio of fibrosis in groups A and B was higher than that in groups C and group D 30 days after modeling (P < 0.001). The number of endometrial glands and MVD in group A was significantly lower than those in groups B, C and D (P < 0.05). The pregnancy rate in group A was 20%, which was lower than that in groups B (33.3%), C (89%) and D (100%) (P < 0.05).
Conclusion: Full-thickness endometrial excision has a high rate of success in constructing stable and effective IUA models in rats.
(© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
Databáze: MEDLINE
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