DNA storage in thermoresponsive microcapsules for repeated random multiplexed data access.

Autor: Bögels BWA; Laboratory of Chemical Biology, Department of Biomedical Engineering, Eindhoven University of Technology, Eindhoven, The Netherlands.; Institute for Complex Molecular Systems (ICMS), Eindhoven University of Technology, Eindhoven, The Netherlands.; Computational Biology Group, Department of Biomedical Engineering, Eindhoven University of Technology, Eindhoven, The Netherlands., Nguyen BH; Microsoft, Redmond, WA, USA.; Paul G. Allen School of Computer Science and Engineering, University of Washington, Seattle, WA, USA., Ward D; Paul G. Allen School of Computer Science and Engineering, University of Washington, Seattle, WA, USA., Gascoigne L; Institute for Complex Molecular Systems (ICMS), Eindhoven University of Technology, Eindhoven, The Netherlands.; Laboratory of Self-Organizing Soft Matter, Department of Chemical Engineering and Chemistry, Eindhoven University of Technology, Eindhoven, The Netherlands., Schrijver DP; Laboratory of Chemical Biology, Department of Biomedical Engineering, Eindhoven University of Technology, Eindhoven, The Netherlands., Makri Pistikou AM; Laboratory of Chemical Biology, Department of Biomedical Engineering, Eindhoven University of Technology, Eindhoven, The Netherlands.; Institute for Complex Molecular Systems (ICMS), Eindhoven University of Technology, Eindhoven, The Netherlands.; Computational Biology Group, Department of Biomedical Engineering, Eindhoven University of Technology, Eindhoven, The Netherlands., Joesaar A; Laboratory of Chemical Biology, Department of Biomedical Engineering, Eindhoven University of Technology, Eindhoven, The Netherlands.; Institute for Complex Molecular Systems (ICMS), Eindhoven University of Technology, Eindhoven, The Netherlands.; Computational Biology Group, Department of Biomedical Engineering, Eindhoven University of Technology, Eindhoven, The Netherlands., Yang S; Laboratory of Chemical Biology, Department of Biomedical Engineering, Eindhoven University of Technology, Eindhoven, The Netherlands.; Institute for Complex Molecular Systems (ICMS), Eindhoven University of Technology, Eindhoven, The Netherlands.; Computational Biology Group, Department of Biomedical Engineering, Eindhoven University of Technology, Eindhoven, The Netherlands., Voets IK; Institute for Complex Molecular Systems (ICMS), Eindhoven University of Technology, Eindhoven, The Netherlands.; Laboratory of Self-Organizing Soft Matter, Department of Chemical Engineering and Chemistry, Eindhoven University of Technology, Eindhoven, The Netherlands., Mulder WJM; Laboratory of Chemical Biology, Department of Biomedical Engineering, Eindhoven University of Technology, Eindhoven, The Netherlands.; Department of Internal Medicine and Radboud Center for Infectious Diseases (RCI), Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands., Phillips A; Microsoft Research, Cambridge, UK., Mann S; Centre for Protolife Research and Centre for Organized Matter Chemistry, School of Chemistry, University of Bristol, Bristol, UK.; School of Materials Science and Engineering, Shanghai Jiao Tong University, Shanghai, People's Republic of China.; Zhangjiang Institute for Advanced Study (ZIAS), Shanghai Jiao Tong University, Shanghai, People's Republic of China., Seelig G; Paul G. Allen School of Computer Science and Engineering, University of Washington, Seattle, WA, USA.; Department of Electrical Engineering, University of Washington, Seattle, WA, USA., Strauss K; Microsoft, Redmond, WA, USA.; Paul G. Allen School of Computer Science and Engineering, University of Washington, Seattle, WA, USA., Chen YJ; Microsoft, Redmond, WA, USA. yuanjc@microsoft.com.; Paul G. Allen School of Computer Science and Engineering, University of Washington, Seattle, WA, USA. yuanjc@microsoft.com., de Greef TFA; Laboratory of Chemical Biology, Department of Biomedical Engineering, Eindhoven University of Technology, Eindhoven, The Netherlands. t.f.a.d.greef@tue.nl.; Institute for Complex Molecular Systems (ICMS), Eindhoven University of Technology, Eindhoven, The Netherlands. t.f.a.d.greef@tue.nl.; Computational Biology Group, Department of Biomedical Engineering, Eindhoven University of Technology, Eindhoven, The Netherlands. t.f.a.d.greef@tue.nl.; Institute for Molecules and Materials, Radboud University, Nijmegen, The Netherlands. t.f.a.d.greef@tue.nl.; Center for Living Technologies, Eindhoven-Wageningen-Utrecht Alliance, Utrecht, The Netherlands. t.f.a.d.greef@tue.nl.
Jazyk: angličtina
Zdroj: Nature nanotechnology [Nat Nanotechnol] 2023 Aug; Vol. 18 (8), pp. 912-921. Date of Electronic Publication: 2023 May 04.
DOI: 10.1038/s41565-023-01377-4
Abstrakt: DNA has emerged as an attractive medium for archival data storage due to its durability and high information density. Scalable parallel random access to information is a desirable property of any storage system. For DNA-based storage systems, however, this still needs to be robustly established. Here we report on a thermoconfined polymerase chain reaction, which enables multiplexed, repeated random access to compartmentalized DNA files. The strategy is based on localizing biotin-functionalized oligonucleotides inside thermoresponsive, semipermeable microcapsules. At low temperatures, microcapsules are permeable to enzymes, primers and amplified products, whereas at high temperatures, membrane collapse prevents molecular crosstalk during amplification. Our data show that the platform outperforms non-compartmentalized DNA storage compared with repeated random access and reduces amplification bias tenfold during multiplex polymerase chain reaction. Using fluorescent sorting, we also demonstrate sample pooling and data retrieval by microcapsule barcoding. Therefore, the thermoresponsive microcapsule technology offers a scalable, sequence-agnostic approach for repeated random access to archival DNA files.
(© 2023. The Author(s).)
Databáze: MEDLINE
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