Dynamic changes of the Prf/Pto tomato resistance complex following effector recognition.

Autor: Sheikh AH; School of Life Sciences, University of Warwick, Coventry, CV4 7AL, UK.; Center for Desert Agriculture, BESE Division, King Abdullah University of Science and Technology, Thuwal, 23955-6900, Saudi Arabia., Zacharia I; School of Life Sciences, University of Warwick, Coventry, CV4 7AL, UK., Pardal AJ; School of Life Sciences, University of Warwick, Coventry, CV4 7AL, UK., Dominguez-Ferreras A; School of Life Sciences, University of Warwick, Coventry, CV4 7AL, UK., Sueldo DJ; School of Life Sciences, University of Warwick, Coventry, CV4 7AL, UK.; Department of Biology, Faculty of Natural Sciences, Norwegian University of Science and Technology, Hogskoleringen 1, 7491, Trondheim, Norway., Kim JG; Department of Biology, Stanford University, Stanford, CA, 94305, USA., Balmuth A; J.R. Simplot Company, Boise, ID, USA.; The Sainsbury Laboratory, Norwich Research Park, Norwich, NR4 7UH, UK., Gutierrez JR; The Sainsbury Laboratory, Norwich Research Park, Norwich, NR4 7UH, UK., Conlan BF; Research School of Biology, The Australian National University, Acton, 2601, ACT, Australia., Ullah N; School of Life Sciences, University of Warwick, Coventry, CV4 7AL, UK., Nippe OM; School of Life Sciences, University of Warwick, Coventry, CV4 7AL, UK., Girija AM; School of Plant Sciences and Food Security, Tel-Aviv University, 69978, Tel-Aviv, Israel., Wu CH; The Sainsbury Laboratory, Norwich Research Park, Norwich, NR4 7UH, UK., Sessa G; School of Plant Sciences and Food Security, Tel-Aviv University, 69978, Tel-Aviv, Israel., Jones AME; School of Life Sciences, University of Warwick, Coventry, CV4 7AL, UK., Grant MR; School of Life Sciences, University of Warwick, Coventry, CV4 7AL, UK., Gifford ML; School of Life Sciences, University of Warwick, Coventry, CV4 7AL, UK.; Warwick Integrative Synthetic Biology Centre, University of Warwick, Coventry, CV4 7AL, UK., Mudgett MB; Department of Biology, Stanford University, Stanford, CA, 94305, USA., Rathjen JP; Research School of Biology, The Australian National University, Acton, 2601, ACT, Australia., Ntoukakis V; School of Life Sciences, University of Warwick, Coventry, CV4 7AL, UK. v.ntoukakis@warwick.ac.uk.; Warwick Integrative Synthetic Biology Centre, University of Warwick, Coventry, CV4 7AL, UK. v.ntoukakis@warwick.ac.uk.
Jazyk: angličtina
Zdroj: Nature communications [Nat Commun] 2023 May 04; Vol. 14 (1), pp. 2568. Date of Electronic Publication: 2023 May 04.
DOI: 10.1038/s41467-023-38103-6
Abstrakt: In both plants and animals, nucleotide-binding leucine-rich repeat (NLR) immune receptors play critical roles in pathogen recognition and activation of innate immunity. In plants, NLRs recognise pathogen-derived effector proteins and initiate effector-triggered immunity (ETI). However, the molecular mechanisms that link NLR-mediated effector recognition and downstream signalling are not fully understood. By exploiting the well-characterised tomato Prf/Pto NLR resistance complex, we identified the 14-3-3 proteins TFT1 and TFT3 as interacting partners of both the NLR complex and the protein kinase MAPKKKα. Moreover, we identified the helper NRC proteins (NLR-required for cell death) as integral components of the Prf /Pto NLR recognition complex. Notably our studies revealed that TFTs and NRCs interact with distinct modules of the NLR complex and, following effector recognition, dissociate facilitating downstream signalling. Thus, our data provide a mechanistic link between activation of immune receptors and initiation of downstream signalling cascades.
(© 2023. The Author(s).)
Databáze: MEDLINE
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