Characterization of transitional memory CD4+ and CD8+ T-cell mobilization during and after an acute bout of exercise.
| Autor: | Hunt RM; Laboratory of Integrated Physiology, Department of Health and Human Performance, University of Houston, Houston, TX, United States., Elzayat MT; Laboratory of Integrated Physiology, Department of Health and Human Performance, University of Houston, Houston, TX, United States., Markofski MM; Laboratory of Integrated Physiology, Department of Health and Human Performance, University of Houston, Houston, TX, United States., Laughlin M; Houston Methodist Orthopedics and Sports Medicine, Houston Methodist, Houston, TX, United States., LaVoy EC; Laboratory of Integrated Physiology, Department of Health and Human Performance, University of Houston, Houston, TX, United States. |
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| Jazyk: | angličtina |
| Zdroj: | Frontiers in sports and active living [Front Sports Act Living] 2023 Apr 17; Vol. 5, pp. 1120454. Date of Electronic Publication: 2023 Apr 17 (Print Publication: 2023). |
| DOI: | 10.3389/fspor.2023.1120454 |
| Abstrakt: | T-cell subsets, including naïve (NA), central memory (CM), transitional memory (TM), effector memory (EM), and RA + effector memory (EMRA), differ in phenotype and function. T-cells are mobilized by exercise, with differences in the magnitude of mobilization between subsets. However, the response of TM T-cells to exercise has not yet been described. Further, T-cells expressing the late differentiation marker CD57 are known to be highly responsive to exercise, but the relative response of CD57 + and CD57- within T-cell subsets is unknown. We therefore aimed to characterize the exercise-induced mobilization of TM T-cells, as well as to compare the exercise response of CD57 + and CD57- cells within T-cell subsets. Methods: Seventeen participants (7 female; aged 18-40 years) cycled 30 min at 80% of their estimated maximum heart rate. Venous blood obtained pre, post, and 1H post-exercise was analyzed by flow cytometry. CD45RA, CCR7, and CD28 expression within CD4 + and CD8+ T-cells identified NA, CM, TM, EM, and EMRA subsets. CD57 expression within EM, EMRA, and CD28+ T-cells was also quantified. The relative mobilization of each subset was compared by calculating fold change in cell concentration during (ingress, post/pre) and after exercise (egress,1H post/post). Cytomegalovirus (CMV) serostatus was determined by ELISA and was considered in models. Results: TM CD8+ T-cell concentration was greater post-exercise than pre-exercise (138.59 ± 56.42 cells/µl vs. 98.51 ± 39.68 cells/µl, p < 0.05), and the proportion of CD8 + with a TM phenotype was elevated 1H post-exercise (1H: 32.44 ± 10.38% vs. Pre: 30.15 ± 8.77%, p < 0.05). The relative mobilization during and after exercise of TM T-cells did not differ from NA and CM but was less than EM and EMRA subsets. Similar results were observed within CD4+ T-cells. CD57 + subsets of CD28+ T-cells and of EM and EMRA CD8+ T-cells exhibited a greater relative mobilization than CD57- subsets (all p < 0.05). Conclusion: These results indicate TM CD4 + and CD8+ T-cells are transiently mobilized into the blood with exercise, but not to as great of an extent as later differentiated EM and EMRA T-cells. Results also indicate CD57 identifies highly exercise responsive cells within CD8+ T-cell subsets. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (© 2023 Hunt, Elzayat, Markofski, Laughlin and LaVoy.) |
| Databáze: | MEDLINE |
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