CkP1 bacteriophage, a S16-like myovirus that recognizes Citrobacter koseri lipopolysaccharide through its long tail fibers.

Autor: Oliveira H; Centre of Biological Engineering, University of Minho, Braga, Portugal. hugooliveira@deb.uminho.pt.; LABBELS -Associate Laboratory, Braga, Guimarães, Portugal. hugooliveira@deb.uminho.pt., Santos S; Centre of Biological Engineering, University of Minho, Braga, Portugal.; LABBELS -Associate Laboratory, Braga, Guimarães, Portugal., Pires DP; Centre of Biological Engineering, University of Minho, Braga, Portugal.; LABBELS -Associate Laboratory, Braga, Guimarães, Portugal., Boeckaerts D; Department of Biotechnology, Ghent University, Ghent, Belgium., Pinto G; Centre of Biological Engineering, University of Minho, Braga, Portugal.; LABBELS -Associate Laboratory, Braga, Guimarães, Portugal., Domingues R; Centre of Biological Engineering, University of Minho, Braga, Portugal.; LABBELS -Associate Laboratory, Braga, Guimarães, Portugal., Otero J; Centre of Biological Engineering, University of Minho, Braga, Portugal.; Departament de Genètica I de Microbiologia, Universitat Autònoma de Barcelona, Barcelona, Spain., Briers Y; Department of Biotechnology, Ghent University, Ghent, Belgium., Lavigne R; Department of Biosystems, KU Leuven, Louvain, Belgium., Schmelcher M; Institute of Food, Nutrition and Health, ETH Zurich, Zurich, Switzerland., Dötsch A; Max Rubner-Institute, Department of Physiology and Biochemistry of Nutrition, Karlsruhe, Germany., Azeredo J; Centre of Biological Engineering, University of Minho, Braga, Portugal. jazeredo@deb.uminho.pt.; LABBELS -Associate Laboratory, Braga, Guimarães, Portugal. jazeredo@deb.uminho.pt.
Jazyk: angličtina
Zdroj: Applied microbiology and biotechnology [Appl Microbiol Biotechnol] 2023 Jun; Vol. 107 (11), pp. 3621-3636. Date of Electronic Publication: 2023 May 03.
DOI: 10.1007/s00253-023-12547-8
Abstrakt: Citrobacter koseri is an emerging Gram-negative bacterial pathogen, which causes urinary tract infections. We isolated and characterized a novel S16-like myovirus CKP1 (vB_CkoM_CkP1), infecting C. koseri. CkP1 has a host range covering the whole C. koseri species, i.e., all strains that were tested, but does not infect other species. Its linear 168,463-bp genome contains 291 coding sequences, sharing sequence similarity with the Salmonella phage S16. Based on surface plasmon resonance and recombinant green florescence protein fusions, the tail fiber (gp267) was shown to decorate C. koseri cells, binding with a nanomolar affinity, without the need of accessory proteins. Both phage and the tail fiber specifically bind to bacterial cells by the lipopolysaccharide polymer. We further demonstrate that CkP1 is highly stable towards different environmental conditions of pH and temperatures and is able to control C. koseri cells in urine samples. Altogether, CkP1 features optimal in vitro characteristics to be used both as a control and detection agent towards drug-resistant C. koseri infections. KEY POINTS: • CkP1 infects all C. koseri strains tested • CkP1 recognizes C. koseri lipopolysaccharide through its long tail fiber • Both phage CkP1 and its tail fiber can be used to treat or detect C. koseri pathogens.
(© 2023. The Author(s).)
Databáze: MEDLINE
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